蜂窝状聚氨酯支架的制备及性能评价

Fabrication and Evaluation of Polyurethane Scaffolds with Honeycomb-Like Structure

以2，2’-二硫代二乙醇为引发剂，经开环聚合法合成聚8-己内酯三元醇，该大分子二元醇再与1，6-己二异氰酸酯加成聚合得到聚酯型聚氨酯，利用红外光谱仪、差示扫描量热仪和X射线衍射仪对合成聚氨酯结构和性能进行表征；然后，利用生物模板法制备出蜂窝状聚氨酯支架，分别用扫描电子显微镜、基于阿基米德原理的测量方法和万能材料试验机表征支架形貌、孔隙率和压缩性质；最后，通过小鼠成骨细胞MC3T3-E1与支架共培养法评价支架细胞相容性。结果表明，合成聚氨酯分子结构得到验证，呈半结晶性特点；所得聚氨酯支架呈现典型的蜂窝状孔结构，孔径范围为100～140μm，孔隙率为77．2％±4．1％，压缩模量为299．02kPa；肌动蛋白染色和死活细胞染色结果说明该蜂窝状聚氨酯支架能支持MC3T3-E1细胞黏附、生长和增殖，细胞形态良好。该蜂窝状聚氨酯支架在骨组织工程中具有良好的应用前景。

In this study, poly （ε-caprolactone） diol was synthesized via ring-opening polymerization of ε-caprolactone using 2,2＇-dithiodiethanol as an initiator, and then polyurethane was synthesized through addition polymerization of poly （ε-caprolactone） and hexamethylene diisocyanate. Polyurethane was characterized by FTIR, XRD and DSC. Polyurethane scaffolds were fabricated by biotemplating technique, and their porous structure, porosity and mechanical properties were characterized by SEM, archimedes principle based method and mechanical test, respectively. MC3T3-E1 cells were cultured on polyurethane scaffolds to evaluate their cytocompatibility. The results indicated that polyurethane scaffolds had a honeycomb-like channels with a diameter range of 100 μm to 140 μm, and their porosity and compressive modulus were 77.2% ± 4. 1% and 299.02 kPa, respectively. Immunofluorescence staining and live/dead staining results indicated that MC3T3-E1 cells could adhere and proliferate on the scaffolds. The results suggested that the honeycomb-like polyurethane scaffolds were a promising candidate for bone tissue engineering.