185株白念珠菌25S rDNA基因型分布特点

Genotype Distribution of 185 Candida albicans 25S rDNA

目的:研究临床不同来源白念珠菌25S rDNA基因型分布特点,探讨白念珠菌不同基因型与感染部位或不同感染类型之间的联系。方法:特异性扩增不同来源的185株白念珠菌25S rDNA基因Ⅰ型内含子序列,再经过琼脂糖凝胶电泳方法对扩增条带进行基因分型,并对不同感染部位、不同感染类型的白色念珠菌的基因型进行比较。结果:185株白念珠菌中,A型122株(65.9%)、B型35株(18.9%)和C型28株(15.1%),未发现D型与E型菌株;外阴阴道念珠菌病(VVC)来源与深部组织来源的白念珠菌25S rDNA基因型差异具有统计学意义(P=0.000);深部组织来源菌株中,艾滋病相关性和非艾滋病相关性白念珠菌基因型差异无统计学意义(P=0.680)。结论:VVC来源和深部组织来源的白念珠菌25S rDNA基因型不同,而深部组织来源的艾滋病相关性和非艾滋病相关性白念珠菌25S rDNA基因型相同。

Objective：To investigate genotype distribution of Candida albicans （C.albicans） 25S rDNA,and to probe the relationship between different genotypes and infection types or parts.Methods：Specific amplication of 185 C.albicans 25S rDNA Type Ⅰ intron sequences,genotyping were carried out after agarose gel electrophoresis,and statistics comparison was conducted on the genotype of C.albicans on different infectious parts or types.Results：185 C.albicans were classified into three genotypes：122 were genotype A （65.9％）,35 were genotype B （18.9％） and 28 were genotype C （15.1％）,but the genotype D and genotype E were not found.There was statistical significance of the differences of C.albicans 25S rDNA genotypes between vulvovaginal candidiasis （VVC） sources and invasive infection sources （P =0.000）,but for the invasive infection sources,there was no significant difference of C.albicans 25S rDNA genotypes among the HIV/AIDS-related and non HIV/AIDSrelated strains （P =0.680）.Conclusions：There are different characteristics between VVC sources and invasive infection sources strains by 25S rDNA genotype,but for the invasive infection sources,the HIV/AIDS-related and non HIV/AIDS-related strains by 25S rDNA genotypes have the similar distribution characteristics.