基于环境污水监测的埃可病毒11型的循环动态变化及其基因特征分析

Temporal Fluctuation and Genetic Characterization of Echovirus 11 Isolates upon Surveillance of Environmental Sewage in Shandong Province,China

为探讨山东省环境污水监测中埃可病毒11型（Echovirus 11,Echo11）的动态变化及分子流行病学特征,本研究对山东省2011~2012年环境污水监测分离到的Echo11毒株进行了VP1区核酸扩增和序列测定,并与1994~2010年山东省急性弛缓性麻痹（Acute flaccid paralysis,AFP）监测系统中Echo11分离株以及国外同型毒株进行同源性分析和系统发生学分析。结果显示：2011~2012年山东省济南市和临沂市共分离到Echo11 94株,其时间分布具有季节性特征,夏秋季高峰明显。分离毒株之间核苷酸和氨基酸同源性分别为89.5%~100.0%和95.4%~100.0%;与原型株（Gregory）之间核苷酸和氨基酸同源性分别为76.6%~79.7%和90.4%~92.5%。山东环境分离株全部属于A基因型,毒株之间核苷酸变异较大,提示山东地区存在多个传播链共循环。本研究描述了山东省环境污水中Echo11分离株的动态变化和遗传特征,结果证明环境污水监测是探索人类肠道病毒动态流行和遗传变异的重要手段。

We wished to analyze the genetic characterization of echovirus 11（Echo 1 l） from samples of envi- ronmental sewage in Shandong Province （China）. The VP1 coding region was typed as the strains were amplified. Phylogenetic analyses on the VP1 sequences from these isolates, strains isolated from AFP ca- ses in the period 1994-2010 and others published in GenBank were conducted. From 2011 to 2012, 94 Ech- oll strains were isolated from samples of environmental sewage in Jinan and Linyi City in Shandong Prov- ince. Numbers of Echoll were seasonal and reached peaks in the summer and autumn in both cities; A- mong these isolates, nucleotide （nt） identities were 89. 5%-100.0% whereas amino acid （aa） identities were 95.4%-100.0%. The nt and aa identities were 76. 6%-79.7% and 90.4%-92. 5% between those strains and the prototype （Gregory） strain of Echo11, respectively. All isolates from Shandong Province were the A genotype and the strains evolved very rapidly, which suggested that several transmission chains was co-circulating. We described the temporal fluctuation and genetic characterization of Echo11 isolates from surveillance of environmental sewage in Shandong Province, thereby providing important information for exploring the dynamic change and genetic variation of circulating human enteroviruses in this Province in China.