电针“三阴交”对膀胱造瘘术后功能亢进模型大鼠膀胱功能的调节作用

Regulation effects of electroacupuncture at“Sanyinjiao”(SP 6)on bladder function in rats with overactive bladder after cystostomy

目的:观察电针"三阴交"干预对膀胱造瘘术后功能亢进(overactive bladder,OAB)模型大鼠尿流动力学的影响,探讨其对膀胱功能的调节机制。方法:将经膀胱造瘘术后的48只成年SD雌性大鼠随机分为空白组(A组)、空白三阴交组(B组)、空白非经穴组(C组)、OAB模型组(D组)、模型三阴交组(E组)、模型非经穴组(F组),每组8只。D、E、F组采用1%醋酸溶液灌注膀胱制造膀胱功能亢进大鼠模型。A组、D组不予其他干预措施;B组、E组针刺双侧"三阴交",待得气后接电针仪;C组、F组:针刺双侧非经穴点,针刺后接电针仪,选用连续波,频率2Hz,留针30min,连续干预5个排尿周期。采用BL-420E+生物机能实验系统测量并记录膀胱压力及尿动力学各项指标的变化。结果:与A组比较,B组膀胱容积、排尿量增加,差异有统计学意义(均P<0.05),C组排尿率增加(P<0.05);B组与C组各指标比较差异无统计学意义(均P>0.05);与D组比较,E组容量压、膀胱容积、逼尿压、排尿量、排尿率均增加(均P<0.05);与F组比较,E组容量压、逼尿压增加(均P<0.05)。结论:电针刺激"三阴交"穴能显著改善膀胱造瘘术后功能亢进大鼠膀胱的排尿功能,但对功能不亢进状态下大鼠膀胱排尿调节能力的改善不明显。

Objective To observe the effects of electroacupuncture （EA） at ＂Sanyinjiao＂ （SP 6） on urody- namics indices in rats with overactive bladder （OAB） after cystostomy, and to explore its regulation mechanism on bladder function. Methods Forty-eight Sprague-Dawley female rats which received cystostomy were randomly di- vided into a blank group （group A）, a blank Sanyinjiao group （group B）, a blank non-acupoint group （group C）, a model group （group D）, a model Sanyinjiao group （group E） and a model non-acupoint group （group F）, 8 rats in each one. The model of OAB was established with 1 % acetic acid solution perfused into the bladder in the group D, group E and group F. No treatment was given to the group A and group D. Acupuncture was applied at bilate- ral ＂Sanyinjiao＂ （SP 6） in the group B and group E, followed by EA after the arrival of qi. Acupuncture was ap- plied at bilateral non-acupoint in the group C and group F, followed by EA with continuous wave, 2 Hz of frequen- cy for 30 min. The treatment was given for continuous 5 urination cycles. The BL-420 E＋ biological function ex- periment system was used to measure and record the changes of indices of bladder pressure and urodynamies. Re- sults Compared with the group A, the bladder capacity and urine output in the group B were significantly in- creased （both P〈0.05）, and the urination rate was increased in the group C （P〈0.05） ; the differences of each index between group C and group B were not statistically significant （all P〉0.05）. Compared with the group D, the capacity pressure, bladder capacity, detrusor pressure, urinary output and urination rate in the group E were all increased （all P〈0.05）. Compared with the group F, the capacity pressure and detrusor pressure in the group E were in- creased （both P〈0. 05）.Conclusion The EA at ＂Sanyinjiao＂ （SP 6） could significantly improve urine function in rats with OAB after cystostomy, but its regulation effect on urination is not obvious in rats with non-OAB.