HPLC法测定华东菝葜中薯蓣皂苷元的含量

Determination of the Diosgenin in Smilax Sieboldii Miq by HPLC

目的 建立华东菝葜药材中成分薯蓣皂苷元的含量测定方法。方法 采用ZORBAX SB-C18（4.6mm×250mm×5μm）色谱柱,乙腈-水（90：10）为流动相,检测波长为203nm,流速为1ml/min,柱温为30℃.结果 薯蓣皂苷元在40.08～400.80 μg/ml浓度范围内与峰面积呈良好线性关系,相关系数r=0.9997,样品回收率为97.56％,RSD =0.59％ （n =6）.结论 该方法简便、准确,结果稳定,可用于华东菝葜中薯蓣皂苷元的测定。

Objective To establish a mothod for determination of diosgenin in Smilax sieboldii Miq.Methods The method was adopted for the determination with ZORBAX SB-C 18 （4.6mm × 250mm × 5 tμm） as chromatographic column,acetonitrile-water （90：10）as mobile phase; the wavelength was 203nm.The flow rate was 1.0ml/min and the column temperature was at 30℃.Results Range of diosgenin were 40.08-400.80μg/ml （r =0.9997）,with average recoveries of 97.56％ （n =6）,RSD =0.59％.Conclusion The method is simple,accurate and stable,which could be used for the quality control of Smilax sieboldii Miq.