白癜风自体培养黑素细胞移植疗效与白细胞介素17、Foxp3的相关性研究

Relationship between the efficacy of autologous cultured melanocyte transplantation and serum levels of interleukin-17 and FoxP3 in patients with vitiligo

目的 探讨白癜风自体培养黑素细胞移植疗效与白细胞介素17、Foxp3的相关性.方法 对40例稳定期寻常型白癜风患者进行自体培养黑素细胞移植,同时收集其外周血,6个月后根据移植效果将收集的血液分为成功组25例,失败组15例.用ELISA的方法测定移植成功与移植失败外周血中白细胞介素17及Foxp3水平,比较移植成功组与失败组血清中白细胞介素17及Foxp3水平的差异.用SPSS 17.0统计软件进行统计分析.结果 移植成功组血清中白细胞介素17（15.29±7.86） ng/L,明显低于移植失败组（43.88±13.02） ng/L,差异有统计学意义.血清中Foxp3的水平,移植成功组（6.08±2.03） ng/L,高于移植失贮组（3.37±1.81） ng/L,两者之间比较,差异有统计学意义.结论 血清白细胞介素17水平升高、Foxp3水平降低与白癜风患者的移植失败有相关性.

Objective To investigate the relationship between the efficacy of autologous cultured melanocyte transplantation and serum levels of interleukin-17 （IL-17） and FoxP3 in patients with vitiligo.Methods Forty patients with stable vitiligo vulgaris were included in this study,and received autologous cultured melanocyte transplantation.Six months after the transplantation,treatment efficacy was evaluated,and patients were classified into the successfully treated group （n =25） and unsuccessfully treated group （n =15）.Peripheral blood was collected from all the patients before the transplantation,and enzyme-linked immunosorbent assay （ELISA） was performed to measure the serum levels of IL-17 and FoxP3.Statistical analysis was done using two independent samples t-test with the SPSS software （version 17.0）.Results The successfully treated patients showed lower serum levels of IL-17 （（15.29 ± 7.86） vs.（43.88 ± 13.02） ng/L,P ＜ 0.05）,but higher serum levels of FoxP3 （（6.08 ± 2.03） vs.（3.37 ± 1.81） ng/L,P ＜ 0.05） than the unsuccessfully treated patients.Conclusion The increased serum IL-17 and decreased serum FoxP3 may contribute to the failure of autologous cultured melanocyte transplantation in patients with vitiligo.