红牛饮料对慢性酒精性肝损伤大鼠肝功能的保护作用

Protective effect of Red Bull drinks against chronic alcoholic injury in rats

目的:通过观察红牛饮料对慢性酒精性肝损伤大鼠肝功能的保护作用,为预防和保护酒精性肝损伤提供实验依据.方法:采用56度白酒灌胃,建立慢性酒精性肝损伤大鼠模型.阳性组给予白酒,空白组给予0.9%生理盐水,实验组给予白酒同时给予相应红牛饮料饮用.实验末测血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、肝组织匀浆测超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malonic dialdehyde,MDA),金属硫蛋白(metallothionein,MT).左肝叶作常规石蜡切片,进行HE染色,观察肝脏病理学变化.结果:不同组间SOD差异有统计学意义(F=5.793,P=0.017),采用LSD法进行两两比较,结果显示空白组SOD明显高于阳性组(0.882U/mgprot±0.044 U/mgprot vs 0.649 U/mgprot±0.154 U/mgprot,P<0.05).实验组SOD明显高于阳性组(0.797 U/mgprot±0.097 U/mgprot vs 0.649 U/mgprot±0.154 U/mgprot,P<0.05).空白组与实验组差异无统计学意义.不同组间ALT、AST、MDA、MT差异均无统计学意义.肝脏病理切片显示阳性组个别肝组织出现肝淤血,不具有说服力.结论:红牛饮料对慢性酒精性肝损伤大鼠肝功能有一定的保护作用.

AIM： To observe whether Red Bull drinks have a protective effect against chronic alcoholic liver injury in rats. METHODS： Chronic alcoholic liver injury was induced in rats by intragastric administration of alcohol （56°）. Rats were divided into three groups and were given alcohol （positive group）, 0.9% saline （control group）, and alcohol plus Red Bull drinks （experimental group）, respec- tively. Serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, and the activities of superoxide dismutase （SOD）, malondialdehyde （MDA） and metallothionein （MT） were detected by the end of the experi- ment. Conventional paraffin section of the leftlobe was stained with hematoxylin-eosin （HE） to observe pathological changes. RESULTS： SOD showed significant differences among the three groups （F = 5.793, P = 0.017）. SOD was significantly higher in the control group than in the positive group （0.882± 0.044 vs 0.649 ±0.154, P 〈 0.05）, and in the experi- mental group than in the positive group （0.797 ± 0.097 vs 0.649 ± 0.154, P 〈 0.05）, although there was no significant difference between the positive group and experimental group. Liver pathology showed congestion in individual liver tissues in the positive group, which was not persuasive. CONCLUSION： Red Bull drinks have an appre- ciated protective effect against chronic alcoholic liver injury in rats.