新疆维汉弥漫大B细胞淋巴瘤中Bcl-6 c-myc基因易位的差异性研究

Difference of Bcl-6 and c-myc gene translocation between Xinjiang Uygur and Han diffused large B-cell lymphoma subtypes

目的:探讨新疆维吾尔族、汉族弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者Bcl-6、c-myc基因易位的差异及其临床意义。方法:采用荧光免疫原位杂交(FISH)方法,对233例DLBCL活体石蜡切片进行Bcl-6、c-myc基因检测。观察Bcl-6、c-myc基因易位与DLBCL患者临床资料的关系,并对不同民族在不同亚型DLBCL中Bcl-6、c-myc基因易位的情况进行比较。结果:233例DLBCL中,Bcl-6基因重排51例,占21.89%;c-myc基因重排39例,占16.74%;Bcl-6基因易位的表达与患者年龄、性别、发病部位、临床分期和LDH水平无显著相关(P>0.05),而与民族、IPI评分、结外侵犯、B症状、DLBCL不同亚型和近期疗效有相关性(P<0.05);c-myc基因易位的表达与患者年龄、性别、发病部位、临床分期、LDH水平和DLBCL不同亚型无明显相关性(P>0.05),而与民族、IPI评分、结外侵犯、B症状和近期疗效有相关性(P<0.05);维、汉不同民族GCB中Bcl-6、c-myc基因易位比较差异均无统计学意义(P>0.05);维、汉不同民族非生发中心活化B细胞(non-GCB)中Bcl-6、c-myc基因易位比较差异有统计学意义(P<0.05)。结论:Bcl-6,C-myc基因易位的表达与维、汉不同民族、IPI评分、结外侵犯、B症状和近期疗效有相关性;维、汉民族non-GCB亚组中Bcl-6、c-myc基因易位存在差异。

Objective：To investigate the clinical significance of Bcl-6, c-myc gene abnormalities in Xinjiang Uygur and Han diffused large B-cell lymphoma （DLBCL） subtypes. Methods：Bcl-6, c-myc gene was detected by fluorescence in situ hybridization in 233 patients with DLBCL . A relationship was observed among Bcl-6, c-myc gene translocation, and clinical data in DLBCL patients. In addition, a difference was observed among Bcl-6, c-myc gene translocation, and different ethnic groups in different subtypes of DLB-CL. Results：Among the 233 patients, 51 cases （21.89%） had rearranged Bcl 6 gene, and 39 cases （16.74%） had rearranged c-myc gene. Bcl-6 gene translocation and expression was related with age, gender, disease location, clinical stage, and LDH levels （P〉0.05）, but was not related with nationality , international prognostic index score, extranodal involvement, B symptoms, DLBCL subtypes, and recent efficacy （P〈0.05）;c-myc gene translocation and expression was related with age, gender, disease location, clinical stage LDH levels, and DLBCL subtypes （P〉0.05）, but was not related with nationality, IPI score, extranodal involvement, B symptoms, and recent efficacy （P〈0.05）. In the Uygur and Han GCB groups, Bcl-6, c-myc gene translocation showed no significant difference （P〉0.05）. By contrast, in the Uygur and Han non-GCB groups, Bcl-6, c-myc gene translocation showed significant difference （P〉0.05）. Conclusion：Bcl-6, C-myc gene translocation was related with age, gender, disease location, clinical stage, and LDH levels. Bcl-6 gene translocation was also correlated with different subtypes of DLBCL.