标本保存方法和时间对血清总二氧化碳结果的影响

Effect of sample storage method and time on the test results for serum total carbon dioxide

目的研究不同的标本保存方法和时间对血清总二氧化碳(total carbon dioxide,TCO2)检测结果的影响。方法保证TCO2检测系统稳定性符合实验室质量目标的前提下,利用高值QC(quality control)、低值QC、混合血清3种样本,分别检测室温敞口、室温封口、4℃敞口、4℃封口时不同时间点TCO2的浓度,计算TCO2浓度随时间的变化率。结果高值QC、低值QC、混合血清在室温敞口保存75 min时,TCO2结果分别下降了9.65%、13.42%、7.74%;4℃敞口保存75 min时,TCO2结果分别下降了8.96%、8.60%、3.94%;在室温封口保存90 min时,TCO2结果分别下降了4.53%、9.43%、3.94%;在4℃封口保存2 h,TCO2结果仅分别下降了3.44%、3.56%、2.19%。结论随着标本保存时间的延长血清TCO2浓度不断下降,并且不同的保存方法下降的速率不同。封口保存TCO2结果下降低于敞口状态,保存温度越低TCO2结果下降越慢。

Objective To study the effect of sample storage method and time on the test results of serum total carbon dioxide . Methods Under the condition that total carbon dioxide （ TCO2 ） measurement system can guarantee the stability of TCO 2 results.TCO2 of two quality controls and a mixed serum was measured promptly at different time points after storage under various conditions ：open samples at room temperature（25±2）℃, sealed samples at room temperature , open samples at 4 ℃, and sealed samples at 4 ℃.Results High level quality control（QC）, low level QC and mixed serum samples decreased by 9.65%, 13.42%and 7.74%, respectively, at 75 min when samples were stored at room temperature and kept open .The same samples decreased by 4.53, 9.43 and 3.94% at 90 min when stored at room temperature and kept seal .The samples decreased by 8.96, 8.60 and 3.94% at 75 min when stored at 4 ℃ and kept open, and decreased by 3.44%, 3.56%and 2.19% at 2 h when stored at 4 ℃ and kept sealed.Conclusion TCO2 decreased over time and decreased at different rate according to storage condition .TCO2 of sealed samples decreased much slower than open samples .The colder samples stored , the slower TCO 2 decreased .