HER-2阴性乳腺癌中VEGF、COX-2的表达及相关性研究

Expression and correlation of VEGF and COX- 2 in HER- 2 negative breast cancer

目的:研究血管内皮生长因子(VEGF)、环氧化酶-2(COX-2)在91例HER-2阴性乳腺癌组织中的表达及相互关系,分析其与临床病理特征及预后的关系。方法:采用免疫组化SABC法,检测91例HER-2阴性乳腺癌组织中VEGF、COX-2蛋白的表达,并与临床病理特征进行相关性分析。结果:HER-2阴性乳腺癌组织中VEGF阳性表达率为94.5%,其中47.2%为高表达(+++),VEGF的表达与肿瘤大小、淋巴结转移相关(P<0.05)。HER-2阴性乳腺癌组织中COX-2阳性表达率为86.8%,其中34.0%为高表达(+++),COX-2的表达与患者年龄、组织学分级、肿瘤大小、淋巴结转移及TNM分期无关(P>0.05)。VEGF与COX-2的表达呈正相关(r=0.1798,P=0.018),VEGF/COX-2均高表达的患者淋巴结转移率高。结论:VEGF、COX-2在HER-2阴性乳腺癌组织中均高表达且两者呈正相关,联合检测VEGF、COX-2的表达对HER-2阴性乳腺癌预后判断可能具有重要价值。

Objective：To study the expression of vascular endothelial growth factor（ VEGF）and cyclooxygenase-2（COX-2）in HER-2 negative breast cancer tissues of 91 patients and analyze the correlation with clinicopatho-logic features and prognosis. Methods：The expression of VEGF and COX-2 proteins in HER-2 negative breast cancer tissues of 91 patients were detected using immunohistochemistry SABC method. The relationship between COX-2 and VEGF expression was analyzed,together with clinicopathologic features. Results：The positive expression rate of VEGF was 94. 5% in the 91 HER-2 negative breast cancer tissues. The overexpression（score 3＋）rate of VEGF was 47. 2%. The overexpression of VEGF was related with tumor size and lymphatic metastasis（P〈0. 05）. The posi-tive expression rate of COX-2 was 86. 8% in the 91 HER-2 negative breast cancer tissues. The overexpression （score 3＋）rate of COX-2 was 34. 0%. The expression of COX-2 was no correlation with age,histopathological grade,tumor size,lymphatic metastasis and TNM stage（P〉0. 05）. The expression of VEGF was related to the ex-pression of COX-2（r=0. 1798,P=0. 018）. Overexpression VEGF/overexpression COX-2 tumors were proved to have high lymphatic metastasis. Conclusion：In HER-2 negative breast cancer tissues,VEGF and COX-2 were both highly expressed and they showed directly correlation. The expression of VEGF and COX-2 might have great signifi-cance in evaluating prognosis of HER-2 negative breast cancer.