紧密连接蛋白Claudin-1与变应性鼻炎发病机制相关性初探

Correlation of Claudin-1 and nosogenesis in allergic rhinitis

目的研究紧密连接蛋白Claudin-1在变应性鼻炎(AR)患者鼻黏膜中的表达,初步探讨Claudin-1在AR发病机制中的作用。方法选取AR患者32例作为实验组,同时以22例单纯鼻中隔偏曲患者作为对照组。取患者下鼻甲黏膜,行苏木素-伊红(HE)染色,检测鼻黏膜嗜酸性粒细胞(Eos)浸润数量;采用免疫组织化学法检测Claudin-1的表达,比较2组间Claudin-1的表达强度;对2组病例中黏膜Eos数量与鼻黏膜Claudin-1的表达强度进行相关性分析。结果实验组鼻黏膜Eos计数(11.52±3.60)显著高于对照组(1.68±1.36),2组差异有统计学意义(P<0.05)。Claudin-1在下鼻甲黏膜的阳性表达物质呈棕黄色、细颗粒状,定位于黏膜、腺体上皮的细胞膜和细胞质。实验组鼻黏膜中Claudin-1的阳性率(25.00%)较对照组低(81.82%),2组差异有统计学意义(P<0.05)。Claudin-1的表达强度与鼻黏膜Eos数量呈负相关(r=-0.865,P<0.05)。结论Claudin-1在AR患者鼻黏膜中的阳性表达率明显下降,提示其表达下降在AR的发病机制中可能发挥重要的作用。

Objective To investigate the expression of Claudin-1 in allergic rhinitis （ AR ） and the role of Claudin-1 in the pathogenesis of AR. Methods Thirty-two AR patients were recruited as the experimental group and 22 cases with deflection of nasal septum （DNS） as the control group.Specimens of inferior turbinate were fixed by 4%formaldehyde.The number of eosinophils（Eos） was measured by haematoxylin-eosin staining.The expression of Claudin-1 was detected by immunohistochemical staining .The results were compared between the two groups .Correlation of Eos content and the expression intensity of Claudin-1 in nasal mucosa was analyzed .Results Eos counts of the specimens in AR group（11.52 ±3.60） were higher than those in the control group （1.68 ±1.36） （P〈0.05）.The positive findings of Claudin-1 by immunohistochemical analysis in the two groups were found in the cytoplasm of the mucosal epithelial cells and glandular cells （brownish yellow,fine granular）.The positive rate of Claudin-1 in AR group（25.00%） was significantly lower than that in the DNS group （81.82%） （ P〈0.05）.There was negative correlation between the positive rate of Claudin-1 and the Eos count in nasal mucosa （r=-0.865,P〈0.05）.Conclusions The tight junction protein Claudin-1 was expressed in the cytoplasm of the mucosal epithelial cells and glandular cells .The down-regulation of Claudin-1 in nasal muscosa imply that it may play an important role in the pathogenesis of AR.