特比萘芬对24株黑酵母样真菌的体外药敏试验研究

In vitro antifungal susceptibility of terbinafine against 24 strains of black yeast like fungi

目的 评价特比萘芬对7个属24株刺盾霉目（Chaetothyriales）黑酵母样真菌体外敏感性.方法 应用美国国家临床和实验室标准研究所（CLSI）的M38-A2方案.菌悬液终浓度为（0.4 ～5）＆#215;104 CFU/mL,30℃孵育5～7d,测定最低有效浓度（MEC）和最低抑菌浓度（MIC）.结果 特比萘芬对24株黑酵母样真菌MEC范围：0.125 ～4μg/mL,MEC90∶2μg/mL,MEC50∶0.25 μg/mL,GM∶0.392 9 μg/mL,特比萘芬对5株暗色真菌100％生长抑制,MIC范围：1～4 μg/mL,MIC50∶2μg/mL,MIC90∶4μg/mL.结论 特比萘芬对刺盾霉目（Chaetothyriales）中的黑酵母样真菌有较强的抑制作用,50％抑菌作用明显.

Objective To investigate the in vitro susceptibility testing of terbinafine against 24 strains of Black yeast like fungi. Methods CLSI M38-A2 guideline was applied. The media containing the fungi in the concentration of O. 5 ×10^4-4×10^4 CFU/mL were incubated at 30 for 5-7 days. The MIC and MEC values were observed. Results The tested 24 strain of black yeast like fungi were susceptible to terbinafine, MEC range ：0. 125 Ixg/mL-4 txg/mL, MECgo ：2μg/mL, MEC5o ：0.25μg/mL, GM ：0. 392 9μg/mL, Five strains had 100% growth inhibition,MIC range：l-4 μg/mL, MIC5o ：2μg/mL;MICgo ：4 μg/mL. Conclusion Terbinafine is ac- tive aganist black yeast like fungi in order Chaetothyriales. Most strains had MEC which 50% growth inhibition by terbinafine.