黑龙江逊克地区森林革蜱斑点热立克次体DNA的检测

Molecular detection of spotted fever group rickettsia in Dermacentor silvarum from the Xunke Area,China

目的调查黑龙江逊克地区蜱传斑点热自然疫源地,发现该地区蜱携带斑点热立克次体的种类。方法采用斑点热立克次体ompA和gltA基因特异的PCR,检测该地区森林革蜱的DNA样本,并对扩得阳性产物进行测序和聚类分析。结果从60只森林革蜱中检测有14只扩得斑点热立克次体ompA和gltA基因片段,阳性率为23.33%。随机选择2只蜱的阳性片段进行测序,二者同源性为100%,ompA基因序列与Rickettsia sp.JL-02同源性为99.30%,与Rickettsia raoultii为99.18%。结论黑龙江省逊克地区森林革蜱携带与Rickettsiasp.JL-02株亲缘关系相近的斑点热群立克次体。

ABSTRACT：To identify the prevalence and the distribution of ticks infected with spotted fever group rickettsiae （SFGR） in Xunke Area of Heilongjiang Province, China, partial outer membrane protein A gene （ompA） and citrate synthase gene （gltA） specific fragments were assessed using the PCR method. The positive products were sequenced. Result showed that the pres- ence of SFGR was 14 of 60 in detection Dermacentor silvarum cases, while the overall positive rate was 23.33 %. Its nucleotide sequence of ompA showed 99.3 % and 99.18~ similarity with nucleotide sequence of Rickettsia sp. JL-02 and Rickettsia rao- ultii respectively. And the evolutionary positions of SFGR species were similar with Rickettsia montana and Rickettsia massili- ae. It＇s concluded that the nature focus of tick-borne spotted fever did exist in the area of Xunke Area of Heilongjiang Province, China.