白术水提物对酵母朊病毒[PSI^+]治愈作用的定量研究

Curing effects evaluation of rhizoma atractylodis macrocephalae on yeast prion [PSI^+]

目的定量研究白术水提物对酵母朊病毒[PSI+]的治愈作用。方法首先用含白术水提物的培养基培养酵母朊病毒[PSI+]阳性菌株,初步评价白术的治愈作用。然后在细胞水平借助影印培养法和蛋白水平上使用半变性琼脂糖凝胶电泳结合蛋白免疫技术进一步验证白术水提物对酵母朊病毒[PSI+]的治愈作用。结果白术水提物作用酵母朊病毒[PSI+]5d后的治愈率为6%。结论白术水提物对酵母朊病毒[PSI+]有一定的治愈作用。

Investigation of the effects of rhizome atractylodis macrocephalae on yeast prion [PSI^＋]was conducted in this study. Liquid culture containing rhizome atractylodis macrocephalae aqueous extracts was applied to evaluate its therapeutic effects preliminarily. Then yeast replica plating together with Semi Denaturing Detergent Agarose Gel Electrophoresis com- bined with western blot were used to further confirm the results. Its showed that the cure rate of aqueous extracts of rhizome atractylodis macrocephalae on yease prion [PSI^＋]could reach 60%.