摘要
目的:探讨RNA干扰技术(RNAi)沉默环氧化酶-2(COX-2)基因对血管内皮生长因子-C(VEGF-C)的表达及细胞迁移能力的影响。方法:利用siRNA干扰胃癌SGC-7901细胞COX-2基因,RT-PCR和免疫荧光检测干扰前后COX-2基因和VEGF-C基因在mRNA和蛋白水平表达差异;细胞划痕实验比较COX-2基因对细胞迁移能力变化的影响。结果:COX-2基因干扰后,胃癌SGC-7901细胞中COX-2基因和VEGF-C基因的mRNA和蛋白水平明显下调,组间差异具有统计学意义(P<0.05),且COX-2与VEGF-C的表达呈显著正相关性(P<0.05);同时胃癌细胞的迁移能力也明显降低。结论:siRNA能特异性地抑制胃癌SGC-7901细胞COX-2基因的表达,进而抑制VEGF-C的表达,降低胃癌细胞的迁移能力,减少胃癌细胞转移。
AIM. To investigate the effects of using RNA interference to silence the expression of COX-2 gene on the expression of VEGF-C and cell migration. METHODS.. Using siRNA inter ference the COX-2 gene in gastric carcinoma cell line SGC-7901, and RT-PCR and immunofluo- rescence detect the expression of COX-2 and VEGF-C gene,comparison the effect COX-2 gene on cell migration by cell scratch test. RESULTS.COX-2 gene and VEGF-C gene were significantly reduced in mRNA and protein levels after the silence of COX-2 gene in gastric cancer cell line SGC-7901, with statistically significant difference between groups （P〈0.05）. The expression of COX-2 was closely correlated with the expression of VEGF-C （P〈 0.05） ; the mi- gration ability of gastric cancer cells also decreased. CONCLUSION： In gastric carcinoma cell SGC-7901, siRNA can inhibit the COX-2 expression,and COX-2 gene could enhance the migration ability of gastric cancer cells. The silence of COX-2 gene could download the expression of VEGF-C,reduce the migration ability of gastric cancer cells, therefore reducing the metastasis of gastric cancer cell.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2014年第9期961-966,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
甘肃省自然科学基金项目(1107RJZA121)
