iPSCs遗传稳定性与重编程机制的研究进展

Reprogramming mechanism and genetic stability of induced pluripotent stem cells(iPSCs)

诱导性多能干细胞(Induced pluripotent stem cells,iPSCs)是采用特定转录因子,将体细胞重编程为具有多能性的干细胞。iPSCs已成功由多种体细胞诱导出来,不仅具有发育多能性还能避免胚胎干细胞(Embryonic stem cells,ESCs)的伦理道德问题,已成为生命科学领域不可或缺的研究工具,具有广阔的应用前景。但获得高质量、遗传稳定的iPSCs是当前亟须解决的问题。文章对iPSCs重编程机制和遗传稳定性的研究进展进行了综述,以期为提高iPSCs的诱导效率、降低诱导成本、掌握iPSCs质量控制的关键点提供参考,从而推进多能性干细胞临床应用的发展。

Induced pluripotent stem cells （iPSCs） were reprogrammed from somatic cells using specific transcription fac- tors. Bypassing the ethical issue caused by embryonic stem cells （ESCs）, iPSCs can be successfully induced from a variety of cells, which makes iPSCs a powerful research tool for developmental biology, iPSCs have also become indispensable to the research of life science due to their broad potential applications. However, it＇s a big challenge to obtain iPSCs with high qual- ity and genetic stability. Here, we review the research progress of increasing the reprogramming mechanism and genetic sta- bility of iPSCs in order to provide references of reprogramming efficiency of iPSCs, reducing the cost, and addressing key points of iPSCs quality control, further promotingclinical application of the iPSCs.