摘要
目的 探讨miR-451是否通过靶向调控巨噬细胞移动抑制因子(MIF)表达抑制胃癌细胞的迁移和侵袭,从而进一步揭示miR-451的抑瘤机制.方法 运用qRT-PCR检测72例胃癌组织及癌旁正常胃黏膜组织中miR-451的表达改变;采用Western blot检测外源高表达miR-451对MIF蛋白表达水平的影响;然后采用划痕实验和Transwell侵袭实验检测外源高表达miR-451对人胃癌MGC-803细胞迁移和侵袭的影响.结果 qRT-PCR检测结果显示,miR-451在72例胃癌组织中的表达水平较癌旁正常胃黏膜组织明显下调,miR-451的表达水平与胃癌患者的临床分期和淋巴结转移呈负相关;Western blot结果显示,过表达miR-451能下调MIF蛋白的表达.划痕实验和Transwell侵袭实验检测发现,过表达miR-451能抑制MGC-803细胞迁移和侵袭能力,差异均具有统计学意义(P<0.05).结论 miR-451通过靶向调控MIF的表达抑制胃癌细胞的迁移和侵袭.
Objective To explore whether miR-451 suppresses cell migration and invasion by targeting macrophage migration inhibitory factors (MIF),thus to reveal molecular mechanism that miR-451 functions as a tumor suppressor in gastric cancer.Methods A quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to detect the expression of miR-451 in gastric cancer and normal stomach mucosa.Western blotting was performed to detect the expressions of MIF protein regulated by miR-451 in MGC 803 cells.The migration and invasion abilities of MGC-803 cell were evaluated with wound-healing and transwell invasion assays.Results miR-451 was down-regulated in gastric cancer tissues.The expressions of miR-451 were negatively correlated with the clinical stage and lymph node metastasis in gastric cancer patients.Western blot showed that a notable reduction of the protein level of MIF by restoring miR-451 in MGC-803 cells.Overexpression of miR-451 inhibited the migration and invasion of MGC-803 cells.Conclusions miR-451 suppresses cell migration and invasion by targeting MIF in gastric cancer.
出处
《中国医师杂志》
CAS
2014年第9期1172-1175,共4页
Journal of Chinese Physician
关键词
微RNAs
胃肿瘤/病理学
胃肿瘤/遗传学
巨噬细胞游走抑制因子
细胞运动
肿瘤浸润
MicroRNAs
Stomach neoplasms/pathology
Stomach neoplasms/genetics
Macrophage migration-inhibitory factors
Cell movement
Neoplasm invasiveness