广西壮族人脂联素基因多态性与妊娠期糖尿病的关系

The relationship between human adiponectin gene polymorphism of Guangxi Zhuang ethnic group and gestational diabetes mellitus

目的 研究脂联素基因单核苷酸多态性（SNP） ＋45T/G和＋276G/T两个位点与广西地区壮族妇女妊娠期糖尿病的关系.方法 采用聚合酶链反应-限制性片段长度多态性技术对98例妊娠期糖尿病（GDM）孕妇（GDM组）及135例糖耐量正常孕妇（对照组）脂联素基因启动予多态性位点（＋ 45T/G和＋276G/T）进行基因分析.结果 GDM组SNP＋ 45 T/G多态性的TG、GG基因型频率显著高于对照组（P＜0.05）,G等位基因频率显著高于对照组（P＜0.05）,GDM组SNP＋ 276 G/T多态性的基因型频率和等位基因频率与正常对照组比较差异无统计学意义（P＞0.05）.GDM孕妇SNP＋ 45T/G多态性位点TG＋ GG基因型组甘油三酯水平高于TT基因型组（P＜0.05）,血清脂联素水平低于TT基因型组（P＜0.05）.结论 脂联素基因＋45T/G位点多态性可能与GDM的发生有关,G等位基因在GDM孕妇中分布频率增加,T→G突变可能通过降低血清脂联素水平参与GDM的发生.检测孕妇该位点,可预测GDM的发生风险.

Objective To investigate the association of single nuchotide polymorphism （SNP） ＋ 45 T/G and ＋ 276 G/T in the adiponectin gene with type 2 diabetes in Guangxi Zhuang pregnant women.Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method was used to determine the distributions of allele and genotype frequencies of SNP ＋ 45T/G polymorphism and SNP ＋ 276 G/T polymorphism in adiponect in gene in 98 type 2 diabetic patients and 135 normal control subjects.Results The TG,GG genotype frequencies of the SNP ＋45T/G were higher in gestational diabetes mellitus （GDM） than those in controls （P 〈 0.05）.The G-allele frequency was significantly higher than those in controls （P 〈 0.05）.The distribution of genotype and allele frequencies of the SNP ＋ 276 G/T polymorphism was not different in diabetes and control groups （P 〉 0.05）.Women carrying the TG and GG allele of SNP ＋45T/G showed higher TG （P 〈0.05）,and lower APN levels than those with TT genotype （P 〈0.05）.Conclusions G allele of SNP ＋45T/G was related to GDM possibly.T→G variation might involve in the occurrence of GDM by reducing serum diponectin levels.Monitoring this site in pregnant women could predict the risk of GDM.