有氧运动对中老年大鼠骨髓内皮祖细胞增殖能力的影响

Effects of Aerobics on Proliferative Capacity in Endothelial Progenitor Cells from Bone Marrow of Old Male Rats

目的:探讨有氧运动对中老年大鼠骨髓来源内皮祖细胞增殖能力的影响。方法:健康中年雄性SD大鼠12只,按体重随机分为两组,即空白对照组和有氧运动组。采用乳酸阈测定法和递增负荷跑台训练建立有氧运动模型,每周训练6天,为期16周。方案结束后12h提取大鼠骨髓,运用密度梯度离心法分离得到单个核细胞,以EGM-2培养诱导其分化获取内皮祖细胞,通过双荧光染色法观察细胞对乙酰化低密度脂蛋白的摄取和与荆豆凝集素1的结合,并结合形态学特征鉴定内皮祖细胞。倒置显微镜下计数贴壁细胞数量和计算集落形成率以反映细胞增殖情况。结果:各组贴壁细胞数量第7d多于第48h(P<0.01);与对照组相比,有氧运动组贴壁细胞数量明显增加(P<0.01),且集落形成率大(P<0.01)。结论:有氧运动能促进中老年大鼠骨髓来源的内皮祖细胞增殖。

Objective： The paper is to explore the effects of aerobics on proliferative capacity in endothelial progeni-tor cells from bone marrow of old male rats. Method： 12 old male Sprague - Dawley rats are randomly and averagely di-vided into 2 groups, the control group and the aerobics group. The aerobic exercise mode is established by using the methods of lactic acid threshold and incremental treadmill exercise. Mononuclear cells from bone marrow are obtained by density gradient centrifugation 12 hours after the scheme has ended up. The cells are suspended in conditioned medium EGM - 2 for culturing in vitro. Their phenotypes are confirmed by uptake of acetylated LDL and binding of fluorescein isothiocyanate （FITC） - labeled Ulex europaeus agglutinin 1 （UEA - 1） lectin. Inverted microscope observation is a-dopted to understand the morphological changes of endothelial progenitor cells and to calculate cells count and colony forming efficiency, so that the proliferation situation of endothelial progenitor cells is obtained. Results： Adherent cells increase more obviously than group control both at 48h and day 7（P 〈 0. 01）. The number of adherent cells and colony forming efficiency in aerobics group is more than that in the control groups（P 〈 0. 01）. Conclusion： Proliferative capaci-ty of endothelial progenitor cells from bone marrow in old male rats can be developed by aerobic exercise.