阿尔泰狗娃花中多糖超声提取及含量测定

RESEARCH ON EXTRACTION PROCESS AND CONTENT DETERMINATION OF HETEROPAPPUS ALTAICUS (WILLD.) NOVOPOKR.POLYSACCHARIDE BY ULTRASONIC EXTRACTION

目的:采用超声法提取阿尔泰狗娃花中的多糖,并测定其含量。方法:采用紫外-可见分光光度法,以葡萄糖为对照品,苯酚-浓硫酸显色法测定阿尔泰狗娃花中多糖的含量。结果:总多糖在2.535-15.21μg/mL范围内呈良好线性关系,回归方程Y=0.0501X-0.0015(r=0.9998,n=6),阿尔泰狗娃花多糖含量为5.645%,RSD为0.65%。经方法学考察显示,稳定性、精密度、重现性均较好,平均回收率为96.86%,RSD为1.45%。结论:紫外分光光度法测定阿尔泰狗娃花中的多糖,操作简便、灵敏度高、重现性好,稳定性高,可作为阿尔泰狗娃花中多糖含量测定方法。

Objective：To extract the polysaccharide of Heteropappus altaicus（ Willd. ） novopokr by ultrasonic extraction, and determine the polysaccharide content. Methods：Based on the standard sample glucose,the polysaccharide content of Heteropappus altaicus （ Willd. ） novopokr polysaccharide was determined by phenol-sulfuric acid coloration and Ultraviolet and Visible（ UV） spectrophotometry. Results：The results showed that the absorbance has a linear relationship with the concentration in the range of 2. 535-15. 21μg/mL,and the regression equation is Y=0. 0501X-0. 0015（r=0. 9998,n=6）. The polysaccharide consent determined is 5. 645% with 0. 65% of RSD. The methodological evaluation demonstrated that this method has good stability, precision and reproducibility. Also, the average recovery rate is 96 . 86%and RSD is 0 . 65%. Conclusion：The UV-spectrophotometry can be employed to determine the polysaccharide content of Heteropappus altaicus（ Willd. ） novopokr,because the method is convenient in operation and has high sensitivity,good repeatability and stability.