5-氮杂胞苷对RASSF1去甲基化与胃癌细胞增殖和凋亡的关系

目的：探讨5-氮杂胞苷对RASSF1基因去甲基化与胃癌细胞增殖和凋亡的关系。方法将SGC7901胃癌细胞分为AZA组（5-氮杂胞苷处理）和CON组（未用5-氮杂胞苷处理），比较两组细胞RASSF1基因启动子甲基化、基因表达、细胞周期和凋亡的差异。结果 CON组SGC7901胃癌细胞RASSF1基因启动子MSP处于甲基化状态，AZA组胃癌细胞未检测到甲基化，AZA组RASSF1基因mRNA和蛋白质表达水平高于CON组细胞。AZA组SGC7901胃癌细胞G0/G1期和凋亡率高于CON组细胞（P〈0.05），S期、G2/M期和PI低于CON组细胞（P〈0.05）。结论5-氮杂胞苷可逆转胃癌细胞RASSF1基因启动子甲基化状态，使沉默的RASSF1基因重新获得表达而抑制胃癌细胞的增殖并促进其凋亡。

Objective To study the effects of 5-azacytidine’s demethylation for RASSF1 gene on gastric cancer cell’s proliferation and apoptosis. Methods SGC7901 gastric cancer cells were divided into CON group（without 5-azacytidine treatment） and AZA group（with 5-azacytidine treatment）. The differences of RASSF1 gene promoter’s methylation status,mRNA and protein expressions,cell cycle and apoptosis were compared. Results The RASSF1 gene was methylated in CON group with inhibited mRNA and protein expressions while they were reversed in AZA group. The S phase、G2/M phase and PI in AZA group were lower than in CONgroup,while the G0/G1 phase and apoptosis rates were higher. Conclusion 5-azacytidine could reverse RASSF1 gene promoter’s methylation and silent gene expressions,which inhibits gastric cancer cell’s proliferation and promote apoptosis.