摘要
目的：探讨癫痫持续状态（status epilepticus，SE）大鼠模型海马区miR-181b表达的变化及意义。方法随机将30只SD（Sprague-Dawley）大鼠分为实验组和对照组，各15只，实验组建立氯化锂-匹罗卡品致SE大鼠模型，对照组以生理盐水代替氯化锂-匹罗卡品，提取两组大鼠右侧海马区脑组织总miRNA和左侧海马组织总蛋白，采用Real-Time RT-PCR检测miR-181b表达及采用Western Blot方法检测caspase-3蛋白的表达变化。结果 SE组大鼠海马区脑组织miR-181b表达较对照组有明显下降（P〈0.05）， caspase-3蛋白表达明显上升（P〈0.05）。结论 miR-181b可能通过调控caspase-3蛋白的表达抑制SE后海马区神经元凋亡过程。
Objective To investigate the expression of miR-181b in the hippocampus of rat with status epilepticus（SE） model. Methods The lithium-pilocapine model of status epilepticus was established in SD rats.The expression of miR-181b and caspase-3 were examined using RT-PCR and western blot separately. Results Compared with the control group,SE group showed decreased expression of miR-181b（P〈0.05）as well as increase in the expression of caspase-3（P〈0.05）in the hippocampus of rat. Conclusion The miR-181b may be involved in the neuronal apoptosis process in rat hippocampus after SE by regulating the expression of caspase-3,which inhibits neuronal apoptosis.
出处
《浙江临床医学》
2014年第10期1544-1546,共3页
Zhejiang Clinical Medical Journal
基金
湖南省科技厅科技计划一般项目（2012FJ3137）
中南大学自由探索计划青年教师助推项目（2012QNZT158）
