热性惊厥患儿外周血NSE和HIF-1α水平变化的临床研究

Clinical study on the changes of peripheral blood NSE and HIF-1αin children with febrile convulsion

目的：观察单纯性热性惊厥（simple febrile convulsion，SFC）患儿不同病程的神经元特异性烯醇化酶（ neuron specific enolase ，NSE）、低氧诱导因子-1α（ hypoxia inducible factor -1α，HIF-1α）的表达水平并探讨其意义，为SFC的临床诊治和预后判断提供科学依据。方法选择SFC患儿53例，分别于惊厥后24、72 h采取静脉血，另选健康对照组33例采血。采用酶联免疫吸附试验（ ELISA）测定血清NSE和HIF-1α水平。结果 SFC患儿NSE和HIF-1α水平明显高于对照组，差异有统计学意义（P＜0．05）；SFC患儿NSE和HIF-1α在惊厥后72 h水平高于24 h水平，但差异无统计学意义（ P＞0．05）。结论外周血NSE和HIF-1α水平的联合测定可作为SFC患儿脑损伤判断的新方法之一。

Objective To observe the expression levels of neuron specific enolase （ NSE ） and hypoxia inducible factor-1α（ HIF-1α） in children with simple febrile convulsion （ SFC） at different disease stages and to explore its clinical significance , so as to provide scientific basis for clinical diagnosis , treatment and judgment of prognosis for SFC . Methods Fifty-three cases of SFC were observed in this study .Venous blood samples were collected at 24 h and 72 h after convulsions .Blood samples from 33 healthy controls （ control group ） were also collected .Serum levels of NSE and HIF-1αwere detected by enzyme -linked immunosorbent assay （ ELISA） .Results NSE and HIF-1αlevels in chil-dren with SFC were significantly higher than that in the control group （P〈0.05）.NSE and HIF-1αlevels in children with SFC were higher at 72 h after the convulsion than at 24 h after the convulsion , but with no statistical significant difference （P〉0.05）.Conclusion Combined determination of peripheral blood NSE and HIF -1αlevels can be one of the new detection method for judging the SFC in children with brain damage .