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切应力作用下联合培养的血管平滑肌细胞对内皮细胞F-肌动蛋白构筑的影响 被引量:5

EFFECT OF CO-CULTURED VASCULAR SMOOTH MUSCLE CELLS ON F-ACTIN CONSTRUCTION OF ENDOTHELIAL CELLS UNDER SHEAR STRESS
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摘要 目的 探讨切应力作用下联合培养的血管平滑肌细胞对内皮细胞抗应力和粘附能力的影响 ,为改进血管内皮细胞种植的组织工程学技术提供生物力学基础。 方法 应用荧光标记和激光共聚焦扫描显微镜技术 ,以静态条件下单独培养的内皮细胞、联合培养的内皮细胞以及切应力作用下单独培养的内皮细胞为对照组 ,研究了切应力作用下与血管平滑肌细胞联合培养的内皮细胞的细胞骨架F 肌动蛋白构筑的变化。 结果 静态条件下单独培养的内皮细胞的F 肌动蛋白排列松散 ,不规则 ,微丝较细 ;联合培养的内皮细胞的F 肌动蛋白微丝明显增多增粗。切应力作用下 ,与血管平滑肌细胞联合培养的内皮细胞的F 肌动蛋白发生重排 ,并形成大量沿切应力方向排列的应力纤维 ,且发生重排的时间明显早于单独培养的内皮细胞。 结论 在切应力作用和血管平滑肌细胞的影响下 ,内皮细胞F Objective The effect of co-cultured vascular smooth muscle cells(VSMCs)on the abilities of against-stress and adhesion of endothelial cells(ECs)under shear stress was studied in order to provide some fundation of biomechanics for improving vascular ECs seeding in tissue engineering. Methods Construction of cytoskeleton-F-actin of ECs co-cultured with VSMCs under shear stress was investigated by the methods of fluorescence-labelling and laser confocal scannin microscopy and ECs cultured alone under the same stress was as a control.Another two controls of ECs cultured alone and co-cultured with VSMCs were maintained under static conditions respectively. Results In static condition,a loose and irregular F-actin construction with its thinner microfiber arose in ECs cultured alone while a regular one with its thicker microfiber arose in ECs co-cultured with VSMCs.Under shear stress,F-actin of ECs co-cultured with VSMCs reconstructed and formed a large amount of stress fiber oriented in the direction of shear stress,and it reconstructed much earlier than ECs cultured alone did.Conclusion Under effect of VSMCs and shear stress,the change of F-actin construction of ECs is of advantage to strengthen the capabilities of against-stress and adhesion of ECs.
出处 《解剖学报》 CAS CSCD 北大核心 2002年第4期380-383,共4页 Acta Anatomica Sinica
基金 国家重点基础研究发展规划 ( 973)资助项目(G2 0 0 0 0 5 6 9 0 5 ) 国家自然科学基金资助项目( 30 0 70 197)
关键词 内皮细胞 平滑肌细胞 联合培养 切应力 F-肌动蛋白 组织工程 Endothelial cells Smooth muscle cells Co-cultured Shear stress F-actin Tissue engineering
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