侧柏内生真菌Chaetomium globosum ZH-32抗菌活性成分研究

Investigation on the antimicrobial ingredients of Chaetomium globosum ZH-32,an endophytic fungus from Platycladus orientalis

采用生物活性追踪法,从侧柏内生真菌Chaetomium globosum ZH-32发酵液中分离得到1个具有抑菌活性的化合物——WH-01,经紫外光谱、核磁共振及质谱等鉴定为球毛壳菌素A。抑菌活性测定结果表明:WH-01对枯草芽孢杆菌Bacillus subtilis、蜡状芽孢杆菌Bacillus cereus、金黄色葡萄球菌Staphylococcus aureus和大肠埃希氏菌Escherichia coli有强烈的抑制作用,其最低抑制浓度(MIC)分别为3.13、3.13、1.56和3.13μg/mL;对番茄灰霉病菌Botrytis cinerea和油菜菌核病菌Sclerotinia sclerotiorum菌丝生长的抑制中浓度(EC50)分别为5.36和12.19μg/mL。

The antimicrobial ingredient （ WH-01 ） of Chaetomium globosum ZH-32 , an endophytic fungus from the leaves of Platycladus orientalis, was obtained by bioassay testing of the extraction from the fermentation broth. The compound was identified as chaetoglobosin A by UV, ESI-MS and NMR techniques, as well as by comparing it with the data published previously. Bioassay results showed that WH-01 had strongly inhibitory activities against Bacillus subtilis, Bacillus cereus, Staphylococcus aureuse, and Escherichia coli, and the minimum inhibitory concentration （ MIC ） values of WH-01 were 3. 13, 3. 13, 1. 56 and 3. 13 μg/mL, respectively. Additionally, WH-01 had excellent inhibitory activities against Botrytis cinerea and Sclerotinia sclerotiorum, and the EC50 values were 5. 36 and 12. 19 μg/mL, respectively.