人胎儿房室结的细胞分类——图像分析定量研究

AN IMAGE ANALYSIS STUDY ON THE CLASSIFICATION OF CELL TYPE WITHIN THE ATRIOVENTRICULAR NODE OF HUMAN FETUS

目的 探讨胎儿房室结内的细胞类型和大小。 方法 石蜡切片 ,H E染色 ,光镜观察胎儿心脏房室结的细胞形态、类型 ;采用HIPAS 10 0 0图像分析仪对其进行定量分析。 结果 胎儿房室结的细胞主要为亮细胞、暗细胞和移行细胞。亮细胞和暗细胞为纺锤形或椭圆形 ,少数多边形 ;移行细胞细短柱形。亮细胞和移行细胞的细胞核明显大于暗细胞。 结论 胎儿房室结的起搏细胞包括亮细胞和暗细胞 。

Objective To investigate cell type and size of fetus atrioventricular node. Methods Using the technique of paraffin section, H-E staining, the cellular morphology and size of atrioventricular node in fetus hearts were observed under microscope, and quantitied by HIPAS-1000 computerized image analytical instrument. Results The cell types of atrioventricular node in fetue hearts were composed of light, dark and transitional cells. The morphology of light and dark cells were spindle-shaped or ellipsoid, but a few cells were polygonal. The shape of transitional cells was short column. The nuclei of light cells and transitional cell were larger than that of dark cells.Conclusion Pacemaker cell of atrioventricular node in fetus heart includes light and dark cells which could present different function types of pacemaker cell. [