香菇纤维素衍生物抑制黄曲霉菌产毒的研究

Study on mushrooms vellulose ferivatives inhibiting aflatoxin production by Aspergillus flavus

黄曲霉毒素具有诱导突变、抑制免疫和致癌作用。控制黄曲霉毒素污染一直是世界性难题,也是近年来的研究热点之一。实验研究了香菇纤维素衍生物GPX抑制黄曲霉菌产毒活性,初步探究了GPX抑制黄曲霉菌产毒的作用机制。结果表明,10、50μg/mL的GPX对黄曲霉菌产毒的抑制率分别达到70%、95%,而100、250、500、750、1000μg/mL的GPX对黄曲霉菌产毒的抑制率均可达到100%,抑制活性极其明显。GPX离体抗氧化能力很低,但是能够缓解黄曲霉菌丝的氧化胁迫。此外GPX促进了黄曲霉细胞内囊泡过早与大液泡的融合,该现象能够抑制黄曲霉毒素的产生。

Aflatoxin is well known with considerable adverse effects including the induction of genetic mutation, liver cancer and the suppression of the immune system on human and animal. Control of aflatoxin contamination is a worldwide problem. Mechanism involved in the aflatoxin-production inhibition by Lentinus edodes cellulose derivatives GPX was investigated. The results showed that inhibition rates of toxin production by 10 and 50μg/mL of the GPX were 70% and 95%,respectively. More GPX（100,250,500,750,1000μg/mL） gave the rise to complete inhibition of toxin production. Without high antioxidant activity in vitro,GPX could modify oxidative stress in A. flavus. In addation,GPX was found to promote premature fusion of vesicles and formation of vacuole in A. flavus,which was proved association with the inhibition of aflatoxin production.