酶解鳙鱼鱼肉蛋白制备ACE抑制肽工艺参数优化

Optimization of hydrolysis process parameters for enzymatic preparation of angiotensin-converting enzyme(ACE) inhibition peptides from bighead carp muscle protein

采用碱性蛋白酶Alcalase 2.4L对鳙鱼鱼肉蛋白进行水解,制备血管紧张素转化酶(ACE)抑制肽。以酶解物ACE抑制率为指标,先进行单因素实验,确定底物浓度([S])、温度、pH、酶用量及水解时间5个因素的最适水平范围,再通过响应面分析法(RSM)对加酶量、温度和水解时间3个酶解工艺参数进行优化。结果表明:Alcalase水解鳙鱼鱼肉蛋白制备ACE抑制肽的最佳酶解条件为:[S]6%、加酶量2.2%、温度57℃,pH9.0及水解时间6.4h,此条件下酶解物ACE抑制率理论值为81.77%,验证实验结果ACE抑制率为81.52%,与理论值相符合。

In order to prepare ACE inhibition peptides with high activity,Alcalase 2.4L was selected to hydrolyze bighead carp（Aristichthys nobilis） muscle protein（BCMP）. The single factor experiment was first used to determine the optimal levels of such five parameters as substrate concentration （[S]）,temperature, pH,enzyme dosage and hydrolysis time based on the ACE inhibitory rate of hydrolyzed BCMP. Then response surface methodology （RSM） was applied to optimize such three parameters as enzyme dosage,temperature and hydrolysis time. The optimal hydrolysis conditions were determined as follows.. [S]6%,enzyme dosage 2.2%, temperature 57℃ ,pH9.0,hydrolysis time 6.4h. Under the hydrolysis conditions,the theoretical value of ACE inhibitory rate of BCMP hydrolysate was 81.77%. The inhibitory rate in the verification experiment was 81.52%, which conformed to the theoretical value.