海带岩藻多糖的提取工艺优化及初步结构分析

Optimization extraction technology and preliminary characterization of fucoidan from Laminaria japonica

采用高压均质与复合酶结合的方法提取海带岩藻多糖,在单因素实验的基础上,选料水比、酶用量、酶作用pH为自变量,以岩藻多糖得率为响应值,利用Box-Behnken实验设计和响应面分析法,优化得岩藻多糖最佳提取工艺条件为:料水比1∶35,酶解pH5.32,酶用量为2.5%,在此条件下,岩藻多糖得率为14.53%±0.05%;利用HPGFC测定岩藻多糖分子量为639、248、79ku;利用IC-PAD检测岩藻多糖的各组分及含量为:岩藻糖42.81%、鼠李糖1.95%、阿拉伯糖1.57%、半乳糖31.35%、葡萄糖3.30%、木糖5.56%、甘露糖13.30%。

Using the high pressure homogeneous combined with composite enzyme extracted fucoidan from Laminaria japonica. With the fucoidan extraction rate as a measure indicator,alga/water ratio,enzyme dosage, pH were optimized by using response surface methodology based on single factor experiments. Then the optimized condition obtained by Box-Behnken design and response surface methodology were as follows. water ratio 1：35, pH5.32,enzyme dosage 2.5%. Under this condition,the extraction rate was 14.53%±0.05%.At last, molecular weight of fucoidan were 639,248,79ku, determined by HPGFC ： IC- PAD analysis showed monosaccharides constituents in the fucoidan were fucose, , rhamnose, arabinose, galactose, glucose, xylose, mannose. And their contents were 42.81%, 1.95%, 1.57%,31.35% ,3.30% ,5.56%, 13.30% respectively.