丹参对AD模型小鼠学习记忆及脑内和血清内乙酰胆碱酯酶含量的影响

Effect of Salvia miltiorrhiza on Learning and Memory and Acetylcholinesterase in Brain and Serum of Mice in AD Model

目的观察丹参对三氯化铝(AlCl3)所致的阿尔兹海默病(AD)模型小鼠学习记忆能力的影响及其脑组织和血清中乙酰胆碱酯酶(AchE)含量的变化。方法 AD模型组:给小鼠每日按400 mg/kg灌胃4%的AlCl3溶液,连续灌胃60 d。正常对照组:每日灌胃与AlCl3等同剂量的生理盐水。丹参治疗组:每日灌胃AlCl3+丹参溶液1 mL。丹参对照组:每日只灌胃丹参溶液1 mL。实验周期为60 d。给药结束后进行Morris水迷宫训练,24 h后进行学习记忆功能测试和乙酰胆碱酯酶含量的测定。结果丹参治疗组、丹参对照组与正常对照组比较,小鼠的逃避潜伏期以及其脑组织和血清中的乙酰胆碱酯酶含量无明显变化,差异无统计学意义(P>0.05)。与模型组小鼠比较,丹参治疗组小鼠的逃避潜伏期明显缩短(P<0.05),并且其脑组织和血清中的乙酰胆碱酯酶含量比模型组明显降低(P<0.05)。结论丹参能有效降低AlCl3致AD模型小鼠脑内及血清中胆碱酯酶活性,明显改善AD模型小鼠的学习记忆功能,这可能是丹参可防治阿尔兹海默病的机制之一。

Objective To evaluate the effects of Salvia miltiorrhiza on the ability of learning and memory and acetyl cholinesterase （AchE） level in brain tissue and serum of aluminium chloride （AlCl3 ） caused AD mouse model. Method The AD mouse model was prepared by intragastric administration of 400 mg/kg AlCl3 for 60 days. Model group （To fill the stomach and AlCl3 equal dose of normal saline. ） Salvia miltiorrhiza treatment group （lavage AlCl3 ＋ Salvia miltiorrhiza 1 mL. ） Salvia miltiorrhiza group（ lavage salvia miltiorrhiza 1 mL）. 24 h After the Morris water maze training, learning and memory function of the mice were tested and the levels of acetyl cholinesterase were measured. Result Salvia miltiorrhiza treatment group and Salvia miltiorrhiza group compared with normal control group, the escape latency in mice and the acetyl cholinesterase levels in the brain tissue and serum no obvious change, there was no statistically significant difference （P 〉 0.05）. The escape latent period in model group, compared with salvia mihiorrhiza treatment group, was prolonged significantly （P 〈 0.05）. The acetyl cholinesterase levels of AD mouse model in brain tissue and serum were higher than other groups （P 〈 0.05 ）. Conclusion Salvia miltiorrhiza can improve the learning and memorizing ability of AlCl3 caused AD model mice and lower the acetyl cholinesterase levels in brain tissue and serum, which are the potential mechanisms of its prevention and treatment effects of alzheimerg disease.