人参皂甙RD预处理对谷氨酸所致PC12细胞损伤的保护作用

Ginsenoside Rd pretreatment attenuates glutamate-induced injury of PC12 cells

目的探讨人参皂甙RD预处理对谷氨酸所致PC12细胞损伤的影响。方法将PC12细胞分为对照组、谷氨酸损伤组和人参皂甙RD预处理组。对照组细胞正常培养;谷氨酸损伤组细胞置于含10 mmon/L谷氨酸的DMEM培养基中培养24 h;人参皂甙RD预处理组细胞经50μmol/L人参皂甙RD预处理30 min后,再加谷氨酸继续培养24 h。采用噻唑蓝比色法检测细胞活力;按试剂盒检测培养液乳酸脱氢酶(LDH)释放量;流式细胞仪检测胞内活性氧(ROS)水平;按试剂盒检测细胞内超氧化物歧化酶(SOD)含量。结果人参皂甙RD预处理最佳浓度为50μmol/L。与谷氨酸损伤组相比,人参皂甙RD预处理PC12细胞活力明显增高(P<0.05),培养液LDH释放量明显降低(P<0.05),胞内ROS含量明显降低(P<0.05),胞内SOD含量明显增高(P<0.05)。结论人参皂甙RD预处理可减轻谷氨酸引起的PC12细胞损伤。

Objective To investigate the effect of ginsenoside Rd on the glutamate（Glu）-induced injury of PC12 cells.Methods PC12 cells were invided into three groups, i.e. control group, Glu group and ginsenoside Rd pretreatment group. PC12 cells in the control group were cultured in Glu-free DMEM medium. PC12 cells in Glu group were exposed in the DMEM medium containing 10 mmol/L Glu for 24 h. PC12 cells in ginsenoside Rd pretreatment group were pretreated with 50 μmol/L ginsenoside Rd for 30 minutes, and then were cultured in DMEM medium containing to 10 m M Glu for 24 h. MTT method was used to determine the cell survival. Lactic dehydrogenase（LDH） level was assessed in the medium to analyze the injury level of the cells. The reactive oxygen species（ROS） level in the medium was determined by flow cytometry. SOD reagent kit was used to determine the intracellular SOD level in the medium. The morphology of PC12 cells was observed by the phase contrast microscope.Results The LDH and ROS levels in the medium which were（857.4±93.6）U/L and（190.2±24.1）% respectively were significantly lower in the ginsenoside Rd pretreatment group than those, which were（1461.0±138.1）U/L and（354.7±48.1）% respectively, in Glu group（P〈0.05）. The SOD level in the medium [（69.5±11.2）%] was significantly higher in ginsesnoside Rd pretreatment group than that [（41.9±6.3）%] in Glu group（P〈0.05）. The cellular morphology was significantly ameliorated in ginsesnoside Rd pretreatment group compared to Glu group.Conclusion Ginsenoside Rd pretreatment can ameliorate PC12 cells injured by Glu.