氯乙烯作业工人血清蛋白质组学研究

The serum proteomics research of vinyl chloride workers

目的 筛选氯乙烯（VCM）接触工人与健康人群血清差异蛋白,分析差异蛋白功能,为寻找VCM接触者的蛋白生物标志提供依据.方法 按照累计接触剂量分别采集VCM接触工人（125人）和健康对照（40人）的空腹静脉血,丙酮沉淀法富集蛋白,2D-nano LC-ESI-TOF/MS鉴定蛋白,相对和绝对定量同位素标记法定量蛋白,用基因本体论分析方法进行差异蛋白功能分析.结果 共鉴定蛋白596种,其中定量蛋白共194种,符合差异蛋白筛选标准的共21种,包括多种补体、载脂蛋白、糖蛋白等,其中上调蛋白19种,下调蛋白2种.主要行使的分子功能为结合作用、酶调节活性、催化活性以及运输活性,主要参与的生物过程包括免疫系统进程和刺激应答.结论 发现了多种补体、载脂蛋白、糖蛋白可能与VCM接触所致肝损伤有关,可以作为VCM接触的蛋白生物标志候选.

Objective To screen out serum differential proteins between vinyl chloride monomer （VCM）-exposed workers and healthy controls by proteomics and analyze the functions of differential proteins,and to provide a basis for elucidating the pathogenesis of diseases caused by VCM exposure and searching for the protein biomarkers.Methods Fasting venous blood was collected from 125 VCM-exposed workers and 40 healthy controls according to accumulated exposure doses.Proteins were precipitated by acetone precipitation.These proteins were identified by 2D-nano LC-ESI-TOF/MS and quantified by isobaric tags for relative and absolute quantitation.The functions of differential proteins were analyzed by gene ontology.Results A total of 596 proteins were identified,including 194 quantified proteins.There were 21 differential proteins according to the screening criteria （19 upregulated proteins and 2 downregulated proteins）,including complement,apolipoprotein,and glycoprotein.The functions of these differential proteins were binding,enzyme regulator activity,catalytic activity,and transporter activity,and they were involved in the biological processes including immune system process and response to stimulus.Conclusion The complement,apolipoprotein,and glycoprotein identified in the proteomics may be related to liver injury caused by VCM exposure,and they could be used as candidate protein biomarkers of diseases caused by VCM exposure.