DMSO与噻酮对离体猪皮组织光学特性参量改变的定量分析

Quantitative Investigation on the Changes of Optical Property Parameters by Using DMSO and Thiazone as Enhancers for Optical Clearing of Porcine Skin in Vitro

从定量的角度评价高渗制剂与组织光学特性参量改变的关系.利用FT-IR红外光谱仪与双积分球系统,对离体猪皮组织在促渗剂二甲基亚砜与噻酮作用下的光谱进行测量,并运用逆倍增方法计算得到组织的光学参量.结果表明:离体猪皮组织的光学特性参量(吸收系数和散射系数)随着高渗制剂作用时间的增加明显减小,光在组织中的穿透深度增加.由此可知猪皮组织的光学特性参量在两种促渗剂作用下随时间发生了动态改变,且噻酮具有比二甲基亚砜更好的清透效果.

The relationship between optical property parameters and the change of hyperosmotic agents were evaluated. Porcine skins, medicated without and with biocompatible hyperosmotic agents-dimethyl sulfoxide and thiazone, were measured by FT-IR spectrophotometer and double-integrating-spheres system. Optical property parameters of samples were calculated by inverse adding-doubling modeling method. The results demonstrated that with the course of the penetration of hiocompatible hyperosmotic agents into porcine skin tissue, optical property parameters （absorption coefficient and scattering coefficient）of tissue samples have got decrease obviously along with the depth of light penetrating into tissue increased. In conclusion, the optical property parameters of bio-tissues could be changed by use of both agents, furthermore, thiazone has led to the better clearing than dimethyl sulfoxide.