涤毒灌肠方对慢性肾衰大鼠粪便吲哚酚与对甲酚含量的影响

Effect of Didu Decoction Enema on Indoxyl and P- cresyl Contents of Feces in Chronic Renal Failure Rats

目的探讨涤毒灌肠方对慢性肾衰大鼠粪便吲哚酚、对甲酚含量的影响。方法 SD大鼠70只,随机抽取8只作为空白对照组,其余62只行5/6肾切除术复制慢性肾衰模型,将造模成功的50只大鼠随机分为病理对照组、涤毒灌肠方大、中、小剂量灌肠治疗组,用药周期为56天。观测大鼠血清肌酐、尿素氮,粪便吲哚酚、对甲酚的含量。结果与模型对照组相比,涤毒灌肠方各剂量治疗组血肌酐、尿素氮明显降低(P<0.05)。灌肠方各剂量组粪便吲哚酚含量显著下降(P<0.01),且大、中剂量组比小剂量组下降更为明显(P<0.05);灌肠方各剂量组粪便对甲酚含量明显下降(P<0.05),各组之间无明显差异。结论涤毒灌肠方减少慢性肾衰大鼠粪便中的吲哚酚、对甲酚产生可能是其干预肾衰竭的作用机制之一。

Objective To study the effect of Didu decoction enema therapy on indoxyl and p- cresyl contents of feces in chronic renal failure rats. Methods： Eight SD rats are randomly divided into control group and 62 SD rats are carried on 5 /6 nephrectomy to set up CRF model. Fifty rats,which are successfully modeling,are randomly divided into pathological control group and Didu enema treatment high dose group,middle dose group and low dose group. After 56 days treatment,the content of creatinine and urea nitrogen of serum,as well as the indoxyl and p- cresyl contents of feces are measured.Results： Compared with the control group,the contents of creatinine and urea nitrogen of serum are significantly decreased in different dose groups of Didu decoction enema treatment（ P 〈 0. 05）. The content of feces indoxyl is significantly decreased（ P 〈 0. 01）. Moreover,the high and middle dose group are better than the low dose group（ P 〈 0. 05）. The significant decreasing of feces p- cresyl content is observed in Didu decoction enema therapy groups（ P 〈 0. 05）. However,there is not significant differences are observed among the groups. Conclusions： Didu decoction enema may play the preventing and treatment role on renal failure by decreasing the content of indoxyl and p- cresyl contents of feces.