姜黄素对脑缺血再灌注损伤大鼠脑组织中NO和S100β水平的影响

Influence of curcumin in NO and S100β levels in brain tissue of rats after focal cerebral ischemia reperfusion injury

目的:观察姜黄素对脑缺血再灌注损伤大鼠脑组织中一氧化氮(NO)和S100β水平的影响,探讨姜黄素对缺血性脑损伤保护作用的机制。方法:成年SD大鼠随机分为假手术组、模型组和姜黄素处理组,每组24只。采用线栓法阻断大脑中动脉(MCAO)2h诱导建立暂时性局部脑缺血模型,姜黄素处理组大鼠术前连续3d给予腹腔注射姜黄素溶液,于脑缺血再灌注后24和72h采用TTC染色观察脑梗死体积,硝酸还原酶法和ELISA法分别检测各组大鼠脑组织中NO和S100β水平。结果:与模型组比较,姜黄素处理组大鼠脑缺血再灌流24和72h时脑梗死体积明显减小(P<0.05)。与假手术组比较,模型组大鼠脑缺血再灌注24和72h时脑组织中NO和S100β水平明显升高(P<0.05);与模型组比较,姜黄素处理组大鼠脑缺血再灌注24和72h时脑组织中NO水平明显降低(P<0.05),脑缺血再灌注72h时脑组织S100β水平明显降低(P<0.05)。结论:姜黄素能明显减轻大鼠脑缺血再灌注损伤的程度,降低脑组织中NO和S100β水平,提示脑组织中NO和S100β水平降低可能与姜黄素的神经保护作用有关。

Objective To observe the influence of curcumin in the levels of nitric oxide （NO） and S100βin the brain tissue of the rats after cerebral ischemia reperfusion, and to explore the neuroprotective mechanism of curcumin. Methods Adult male SD rats were randomly divided into sham operation group, model group and curcumin group, and 18 rats in every group. Cureumin was intraperitoneally injected daily for 3 consecutive days before inducing focal cerebral ischemia in the SD rats by middle cerebral artery occlusion（MCAO） for 2 h. TTC staining was usedto observe the infarction volume. Nitrate reductase assay was used to detect the level of NO in brain tissue of the rats. The level of S10013 in brain was detected by ELISA method. Results Compared with model group, the brain infarction volumes of the rats 24 and 72 h after cerebral ischemia reperfusion in curcumin group were significantly decreased （P〈0. 05）. Compared with sham operation group, the NO and S10013 levels in the brain tissue 24 and 72 h after cerebral ischemia reperfusion of the rats in model group were significantly increased（P〈0. 05） ; compared with model group, the levels of NO in the brain tissue 24 and 72 h after cerebral ischemia reperfusion in curcumin group were remarkably decreased （P〈0. 05） ; compared with modee group, the level of S100β in the brain tissue 72 h after cerebral iscemia reperfusion in curcumin group was remarkably decreased （P 〈 0.05 ）. Conclusion Curcumin can significantly reduce the degree of ischemia reperfusion injury in the rats and reduce the levels of NO and S100β in brain tissue, which suggests that the decrease of NO and S100β levels in brain tissue may be associated with the neuroprotective effect of curcumin.