楤木总皂苷提取物在不同介质中平衡溶解度的测定

Determination of Equilibrium Solubilities of Total Saponins Extract from Aralia chinensis in Different Mediums

目的：测定楤木总皂苷在水,0.1 mol·L^-1盐酸和不同pH磷酸盐缓冲液中的平衡溶解度,为楤木总皂苷提取物的剂型设计提供实验依据.方法：以楤木皂苷A为指标,采用HPLC测定楤木皂苷A在不同介质中平衡溶解度,流动相乙腈（A）-0.1％磷酸溶液（B）梯度洗脱（0 ～ 18 min,35％ ～ 43％ A; 18 ～ 19 min,43％ A; 19～25 min,43％ ～ 35％ A）,检测波长205 nm.结果：37℃时,楤木皂苷A在水,0.1 mol·L^-1盐酸和pH 2,4,6,7,8,10的磷酸盐缓冲液中平衡溶解度分别为1.780,0.241,0.420,0.472,1.196,1.746,0.180,0.242 g·L^-1.结论：楤木总皂苷提取物微溶于水,但受介质的pH影响大.

Objective：To establish a method for determination equilibrium solubilities of total saponins extract from Aralia chinensis in different mediums.Method：Taking araloside A as index,an HPLC method was performed and adopted to detect the concentration of araloside A in water,0.1 mol · L^-1 HC1 and different phosphate buffer with pH of 2,4,6,7,8,10 at 37 ℃,mobile phase of acetonitrile （A）-0.1% phosphoric acid solution （B） for gradient elution （0-18 min,35%-43%A; 18-19 min,43%A; 19-25 min,43%-35% A）,detection wavelength was set at 205 nm.Result：Equilibrium solubilities of araloside A in different mediums were 1.780,0.241,0.420,0.472,1.196,1.746,0.180,0.242 g · L^-1.Conclusion：Total saponins extract from A.chinensis dissolves in water,its equilibrium solubility is influenced greatly by pH values.