HPLC-DAD同时测定白花蛇舌草中2个活性蒽醌类成分

Determination of Two Anthraquinones in Hedyotidis Herba by HPLC-DAD

目的：建立HPLC-DAD测定白花蛇舌草药材中2个蒽醌类成分2-甲基-3-甲氧基蒽醌（蒽醌Ⅰ）、2,3-二甲氧基-6-甲基蒽醌（蒽醌Ⅱ）含量的方法.方法：采用ZORBAX Extend-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.5％磷酸溶液（48∶52）,检测波长265 nm,流速1.0 mL·min^-1,柱温25℃.结果：蒽醌Ⅰ在0.998 ～ 9.98 μg（r=0.999 6）、蒽醌Ⅱ在0.675 ～6.75 μg（r =0.999 9）线性关系良好,平均加样回收率分别为99.87％ （RSD 1.13％）和99.51％ （RSD 0.69％）.不同批次的白花蛇舌草药材中蒽醌Ⅰ和蒽醌Ⅱ成分含量差异较大,蒽醌Ⅰ和蒽醌Ⅱ的比例也无明显规律.结论：该方法简单、准确,适用于白花蛇舌草药材中2-甲基-3-甲氧基蒽醌和2,3-二甲氧基-6-甲基蒽醌的质量控制.

Objective：The aim of this study was to establish an HPLC-DAD method for the determination of 2-methy-3-methoxyanthraquinone,2,3-dimethoxy-6-methyanthraquinone in Hedyotidis Herba.Method：The assay was performed on a ZORBAX Extend-C18 column （4.6 mm × 250 mm,5 μm） eluted with acetonitrile-0.5% phosphate （48∶ 52）.The detection wavelength was 265 nm,the flow rate was 1.0 mL ·min^-1,and the column temperature was set at 25 ℃.Result：The calibration curves were linear in the ranges of 0.998-9.98 μg （r =0.999 6） for anthraquinone Ⅰ and 0.675-6.75 μg （r =0.999 9） for anthraquinone Ⅱ.The average recoveries of anthraquinone Ⅰ and anthraquinone Ⅱ were 99.87% （RSD 1.13%） and 99.51% （RSD 0.69%）,respectively.Conclusion：The method is simple and accurate for the quality control of 2-methy-3-methoxyanthraquinone and 2,3-dimethoxy-6-methyanthraquinone in Hedyotidis Herba.