基于基线等比增减设计法优选葛根-黄连抗消渴最佳配比

Optimization of the Best Ratio of Puerariae Lobatae Radix and Coptidis Rhizoma Against Diabetes Based on Increase-decrease Baseline Geometric Proportion Design Method

目的:通过不同配比葛根-黄连(葛-黄)对不同模型糖尿病小鼠血糖影响,对链尿佐菌素(STZ)复合高脂饲料喂养致糖尿病小鼠模型血清总胆固醇(TC)、甘油三酯(TG)含量的影响,探索葛根-黄连伍用抗消渴的最佳配比方案。方法:雄性SPF级小鼠,对正常小鼠血糖影响实验随机分为空白组、二甲双胍组(0.5 g·kg-1)、葛-黄0∶10,8∶3,6∶5,3∶8,10∶0组(4 g·kg-1);对四氧嘧啶和STZ所致糖尿病小鼠实验,造模72 h后尾尖取血测定小鼠空腹血糖(FBG),成模小鼠随机分为空白组、模型组、二甲双胍组、葛-黄10∶0,8∶3,6∶5,3∶8,0∶10组,给药后7,14 d分别观察不同配比葛-黄对正常小鼠及四氧嘧啶致糖尿病小鼠FBG的影响,对STZ复合高脂饲料致糖尿病小鼠FBG,TC,TG的影响。结果:葛-黄10∶0,8∶3,6∶5,3∶8,0∶10这5组均能明显降低四氧嘧啶致高血糖小鼠模型和STZ复合高脂饲料喂养致糖尿病小鼠模型空腹血糖,降低STZ复合高脂饲料致糖尿病小鼠模型血清TG,TC含量,同时降低正常小鼠空腹血糖,其中降糖、降低TG,TC效果葛-黄3∶8组疗效突出(P<0.01),黄连单味药次之。结论:葛根、黄连单用及伍用抗消渴疗效确切,能明显改善糖尿病相关生化指标,伍用后药效优于单味药且存在最佳配比。

Objective：To observe the influence of different proportions of Puerariae Lobatae Radix （PLR） and Coptidis Rhizoma （CR） on fasting blood glucose （FBG） of different diabetic mice model,and serum triglyceride （TG） and total cholesterol （TC） levels of diabetic mice mode induced by streptozotocin （STZ）,further to explore the best ratio of PLR and CR against diabetes.Method：Male SPF mice were randomly divided into blank control group,metformin group,PLR and CR 10∶0,8∶ 3,6∶ 5,3∶ 8,0∶10 groups; the mice diabetes model weas induced by alloxan and STZ,the tail blood was collected to determine the levels of FBG after 72 hours,the die mice were randomly divided into control group,model group,metformin group,PLR-CR 10∶ 0,8∶ 3,6∶ 5,3∶ 8,0∶10 groups.To observe impact of different proportions of PLR and CR on the FBG of normal mice and alloxan-induced diabetic mice,FBG,TC and TG levels were measured.Result：10∶0,8∶ 3,6∶ 5,3∶ 8,0∶ 10 all groups could significantly reduce the FBG in mice induced by alloxan or STZ,reduce the serum TG and TC levels in mice induced by STZ combined with high fat diet.The group with 3∶8 of PLR to CR had more obvious effect than CR group.Conclusion：The single and combined administration of PLR and CR and have efficacy against diabetes,and could significantly improve diabetes-related biochemical marker.The combine administration is better than single one.