血管内皮生长因子在乳腺癌中的表达及与分子分型的关系

The expression of vascuoar endothelial growth factor and its relationship with molecular subtype in breast cancer

目的：检测血管内皮生长因子（VEGF）在乳腺癌中的表达情况,探讨其与乳腺癌临床病理参数及分子分型之间的关系。方法：采用免疫组织化学法检测乳腺癌标本中VEGF、雌激素受体、孕激素受体、人表皮生长因子受体2（Her-2）及增殖细胞核抗原67的表达情况。对Her-2（2＋）的组织进行荧光原位杂交检测。根据雌激素受体、孕激素受体、Her-2及增殖细胞核抗原67的表达情况,将乳腺癌标本分为4个亚型,检测VEGF在各分子亚型中的表达情况。结果：VEGF在乳腺癌组织组中的阳性率显著高于良性病变组及癌旁组织组（P〈0.01）。VEGF在淋巴结转移者中的阳性率高于无淋巴结转移者（P〈0.05）。VEGF的阳性率均随着组织学分级、临床分期的升高而升高（P〈0.05-P〈0.01）。在不同乳腺癌分子亚型中,Basal-like型乳腺癌组织中VEGF的阳性率高于Luminal A型（P〈0.05）。结论：VEGF参与乳腺癌的发生、发展及浸润转移过程,有可能成为指导临床治疗的重要靶向指标之一。将乳腺癌中VEGF的表达情况与乳腺癌分子分型相互结合,有助于肿瘤恶性程度及预后的判断,有可能成为指导分子分型的又一重要指标。

Objective： To detect the expression of vascuoar endothelial growth factor（ VEGF） and investigate its relationship between clinicopathological features and molecular subtypes in breast cancer. Methods： The expressions of VEGF,estrogen receptor,progesterone receptor,human epidermal growth factor receptor 2（ Her-2） and nuclear associated antigen-67 in breast cancer were detected by immunohistochemical method. The Her-2 gene level was measured by fluorescence in situ hybridization. The breast cancers were divided into four molecular subtypes according to the expression levels of estrogen receptor,progesterone receptor,Her-2 and nuclear associated antigen-67,the expression of VEGF in different subtypes was measured. Results： The positive rate of VEGF expression in breast cancer tissue was significantly higher than that in benign lesion tissue and para-carcinoma tissue（ P〈0.01）. The positive rate of VEGF expression in breast cancer tissue with lymph node metastasis was significantly higher than that in breast cancer tissue without lymph node metastasis（ P〈0.05）. The positive rate of VEGF expression increased with the increasing of histological grade and clinical stage（ P 0.05 to P 0.01）. The positive rate of VEGF expression in type Basal-like was higher than that in type Luminal A（ P 0.05）.Conclusions： VEGF involves the process of occurrence,development and metastasis of breast cancer,which maybe an important index in guiding clinical treatment. Combining the expression of VEGF with molecular subtype of breast cancer can contribute to judge the malignant degree and prognosis of tumor,which maybe another important index in guiding molecular subtype.