黔产东魁杨梅挥发油的气-质联用分析及其抗氧化活性研究

GC-MS Analysis and Antioxidant Activity of Volatile Oil from Myrica Rubra cv.Dongkui Orient Pearl

采用水蒸气蒸馏法从东魁杨梅中提取挥发油,通过气相色谱-质谱联用(GC-MS)技术对其化学成分进行鉴定,用归一化法测定挥发油中各组分的相对百分含量,并采用DPPH·自由基法研究其抗氧化活性。从东魁杨梅果实挥发油中分离出39个峰,鉴定了其中32个,占挥发油总量的93.45%。其主要组分是石竹烯,相对含量为15.48%;其次是8S,14-雪松烯二醇,相对含量为13.54%;相对含量较高的还有棕榈酸(9.98%)、甲酸芳樟酯(6.11%)、β-谷甾醇(5.96%)、乙酸环阿屯酯(4.37%)、环氧化长叶松烯(4.01%)、金合欢醇(3.53%)、蛇麻烯(3.52%)等,同时研究发现东魁杨梅挥发油具有较好的清除DPPH·自由基能力。东魁杨梅挥发油中主要含有萜、甾体、有机酸等多种化学成分,并表现出较好的抗氧化活性,从而进一步揭示了其潜在的开发价值,并为其质量指标的制订提供必要的参考数据。

The volatile oil was extracted by steam distillation and analyzed by GC-MS. The DPPH·method was used to invesitgate the antioxidant activity. Thirty nine chromatographic peaks were detected,among which thirty two compounds have been identified. The contents of them made up 93. 45% of the total composition. The main components of the oil were caryophyllene（ 15. 48%）,8S,14-cedran-diol（ 13. 54%）,n-hexadecanoic acid（ 9. 98%）,linalyl formate（ 6. 11%）,β-sitosterol（ 5. 96%）,cycloartenol acetate（ 4. 37%）,longipinene epoxide（ 4. 01%）,（ E,E）-farnesol（ 3. 53%） and humulene（ 3.52%）. In addition,the oil also showed good antioxidant activity. The results provide scientific basis for quality control of Myrica rubra cv. Dongkui Orient Pearl which are cultivated in Guizhou province.