霉变烟叶和正常烟叶游离氨基酸组成的差异

Differences of Free Amino Acid Components Between Mildewed and Normal Tobacco Leaves

为阐明霉变烟叶和正常烟叶化学组成的差异,用氨基酸分析仪法测定了初烤烟叶中17种游离氨基酸的含量。结果表明:1正常烟叶的游离氨基酸总量是霉变烟叶的3.6倍;2正常烟叶中脯氨酸含量最高,占总量的75.58%;霉变烟叶中含量较高的是天冬氨酸、组氨酸、谷氨酸和苯丙氨酸,四者之和占总量的68.69%;3脯氨酸、天冬氨酸、组氨酸、谷氨酸和苯丙氨酸在两种烟叶中的差异较大。游离氨基酸总量降低以及特定游离氨基酸含量的变化可作为烟叶霉变识别的生物标记。

In order to find out the differences of chemical components between mildewed and normal tobacco leaves, the contents of 17 free amino acids in cured tobacco leaves were determined by an amino acid analyzer. The results showed that： 1） The total amount of free amino acids in normal leaves was 3.6 times of that in mildewed leaves. 2）Proline was the major amino acid and accounted for 75.58% of total amino acids in normal leaves. Whereas the major amino acids in mildewed leaves were aspartic acid, histidine, glutamic acid and phenylalanine, which accounted for 68.69% of total amino acids. 3） The contents of proline, aspartic acid, histidine, glutamic acid and phenylalanine differed more obviously between mildewed and normal leaves. Therefore, the decrease of total amount of free amino acids and the variation of some free amino acids can be used as the biomarkers in mildewed tobacco leaf identification.