花粉管通道和农杆菌介导植酸酶基因phyC对桑树的遗传转化

Genetic Transformation of phy C Gene into Mulberry Mediated by Pollen-tube Pathway and Agrobacterium

植酸酶可以促进单胃动物利用饲料中的植酸磷。桑叶中的含磷量较高,培育转植酸酶基因的桑树品种有利于桑叶饲料的开发利用。构建含中性植酸酶基因phyC的植物表达载体pCAMBIA-2300-phyC,利用花粉管通道结合农杆菌介导的方法将phyC基因导入红果1号×育2号杂交桑种子,1 301粒杂交桑种子经卡那霉素抗性筛选和大田播种共获得154株桑苗,其中11株经PCR鉴定为阳性的phyC基因转化桑苗。该转基因方法具有操作简便、转化范围较广的特点,获得的转phyC基因桑树植株可作为饲料桑品种选育的素材。

Phytase can effectively improve utilization of phytate phosphorus by monogastric animals. Creation and cultivation of mulberry variety with transgenic phytase gene will facilitate development and utilization of mulberry leaf feed since mulberry leaves are rich in phosphorus. An expression vector pCAMBIA-2300-phyC harboring the neutral phytase gene phyC was constructed and transferred into the seed of a hybrid mulberry named Hongguo 1 × Yu 2 by pollen-tube pathway combined with agrobacterium-mediated method. Totally,154 mulberry seedlings survived and grew in the filed from kanamycin resistance selection among 1 301 treated hybrid mulberry seeds,and 11 seedlings were verified as positive phyC-transformed mulberry by PCR analysis. This transgenic method is easy to operate and can be applied in a wide range of plant species. The obtained transgenic mulberry plants harboring phyC gene could be used as breeding materials for forage mulberry varieties.