应用Logistic模型和王-兰-丁模型分析桑丁香假单胞菌突变株M90-1的冠菌素产量提升因素

Analysis on Improvement Factors of Coronatine Production from Mutant Pseudomonas syringae pv. mori Strain M90-1 by Logistic and Wang-Lan-Ding Models

采用常压室温等离子体（ARTP）技术诱变处理桑丁香假单胞菌M4-13,应用Logistic模型和王-兰-丁模型分析突变菌株产冠菌素能力提升的因素,为优化诱变高产菌株培养条件等提供依据。突变菌株M90-1的冠菌素产量达84.604 mg/L,较原始菌株M4-13提高了5.755%。应用Logistic模型建立的菌株生长曲线显示突变菌株M90-1的增殖速度明显下降,平均生长速率较原始菌株降低9.933 3%,但菌株的世代历期和对数生长期时长比原始菌株分别延长24.895 9%、27.506 7%,繁殖代数仅提高2.090 4%;用王-兰-丁模型分析环境温度与菌株生长和冠菌素产能的关系,得出突变菌株M90-1的最适生长温度为32.409 5℃,明显高于原始菌株（24.463 0℃）,突变菌株的高温边界层宽度、温度内禀增长力、高温下潜在饱和增长力、高温发育临界温度分别比原始菌株降低89.492 0%、46.841 3%、8.885 3%和2.606 4%;用时间序列差分稳定性分析方法得到突变菌株1～10代的冠菌素产量稳定性好,变幅在2.241 0%之内。研究结果表明：突变菌株M90-1通过延长生长与发育时间提高冠菌素的产能,可以通过优化菌株培养温度促进其生长和提高冠菌素产量。

The atmospheric room temperature plasma（ ARTP） technology was employed to mutate the Pseudomonas syringae pv. mori M4-13 to improve the yield of coronatine. The improvement factors of coronatine production were analyzed with the Logistic and Wang-Lan-Ding models,which can provide a basis on mutant breeding for high-yield coronatine strains and optimization of culture conditions. The resultant mutant strain M90-1 produced 84. 604 mg /L coronatine that increased by 5. 755% compared with the original strain M4-13. The growth curve of strain M90-1 was analyzed by the Logistic model,and the result showed that the growth rate of M90-1 obviously declined by 9. 933 3% compared with the original strain. However,it prolonged the time of growth and development to compensate in the time dimension： generation duration and logarithmic phase have prolonged by 24. 895 9% and 27. 506 7% respectively.The number of proliferated generations only increased by2. 090 4%. Relationship between ambient temperature with growth of strain and yield of coronatine revealed byWang-Lan-Ding model showed that the optimum growth temperature of mutant strain M90-1 was 32. 409 5 ℃,which was significantly higher than that of the original stain（ 24. 463 0 ℃）. The width of high temperature boundary layer,intrinsic rate of increase,saturated growth potential,and the critical temperature of growth have declined by 89. 492 0%,46. 841 3%,8. 885 3% and 2. 606 4% than that of the original strain respectively. The yield of coronatine was stable from the 1st to 10 th generations of which the amplitude variations were within 2. 241 0% as shown by the finite-difference analysis and time flow analysis. The results indicated that mutant strain M90-1 could extend the growth and development time to improve coronatine production,and it is feasible to improve the growth and coronatine yield of mutant strain M90-1 by optimizing incubation temperature.