粗毛栓菌(Trametes hirsuta)D2固态发酵山核桃蒲壳产漆酶的营养条件研究

Nutritional conditions for laccase production by Trametes hirsuta D2 in solid-state fermentation using hickory hull

【目的】为提高漆酶产量,降低生产成本,以山核桃蒲壳作为基质,对粗毛栓菌D2固态发酵产漆酶的营养条件进行研究。【方法】对不同碳源、氮源、碳氮比、蒲壳含量对漆酶产量的影响进行分析。【结果】山核桃蒲壳是粗毛栓菌生长的良好载体,能够促进漆酶的合成。粗毛栓菌D2漆酶固态发酵培养基干物质组成为:山核桃蒲壳40%(质量比),玉米粉24%(质量比),菜籽饼粉36%(质量比)。发酵6 d时,漆酶活性为126.8 U/g干基。【结论】粗毛栓菌固态发酵山核桃蒲壳产漆酶具有效率高,生产成本低的优点,具有潜在的工业化应用前景。

[Objective] In order to increase the laccase productivity and reduce the production cost,solid-state fermentation（SSF）（using hickory hull） medium for Trametes hirsuta D2 has been studied. [Methods] The effects of supplementation with carbon and nitrogen sources, ratio of carbon and nitrogen source and the content of hickory hull on laccase production were investigated.[Results] The hickory hull was a good support for mycelial growth during laccase fermentation, and the corn flour and rapeseed meal were found efficient and selected as additional nutrition for laccase fermentation. The optimum solid materials in medium were consisted of 40%（W/W） hickory hull,24%（W/W） corn flour and 36%（W/W） rapeseed meal. Under these conditions, the highest laccase activity（126.8 U/g dry substrate） was achieved after 6 days of cultivation. [Conclusion] The higher laccase productivity and lower production cost could facilitate industrial application of laccase.