急性喉气管支气管炎患儿血清中IL-4、IFN-γ和IgE水平的变化及临床意义

Clinical significance of IL-4, IFN-γ and IgE in children with acute laryngotr-acheobronchitis

目的 探讨急性喉气管支气管炎患儿血清IL-4、γ干扰素（IFN-γ）和IgE水平的动态变化及临床意义.方法 采用ELISA双抗体夹心法检测22例急性喉气管支气管炎患儿入院时（急性期）、出院前（临床症状消失）及正常对照组儿童血清IL-4、IFN-γ水平,采用荧光酶联免疫法检测IgE水平,并进行比较.结果 ①急性喉气管支气管炎患儿血清IL-4水平入院时显著高于对照组（P＜0.05）,出院时较入院时明显下降（P＜0.05）,与对照组比较差异无统计学意义（P＞0.05）;②急性喉气管支气管炎患儿血清IFN-γ水平入院时显著低于对照组（P＜0.05）,出院时较入院时明显升高（P＜0.05）,与对照组比较差异无统计学意义（P＞0.05）;③急性喉气管支气管炎患儿血清IgE水平入院时显著高于对照组（P＜0.05）,出院时较入院时明显下降（P＜0.05）,与对照组比较差异无统计学意义（P＞0.05）.结论 急性喉气管支气管炎患儿存在免疫功能紊乱,IL-4、IFN-γ和IgE在患儿免疫病理机制中起重要作用.

Objective To investigate clinical significance and the change of serum IL-4,IFN-γ and IgE levels in infant with acute laryngotracheobronchitis.Methods Serum samples were obtained from 22 children with acute laryngotracheobronchitis,and 25 healthy children.The change of IL-4,IFN-γ and IgE levels in serum when hospital admission （acute stage）and before discharge （the clinical symptom vanished）were observed.ELISA was used to determine the levels of IL-4,IFN-γ in serum.Serum IgE level was determined with enzyme-linked fluoroimmuneassay.Results ①The serum level of IL-4 in patients with acute laryngotracheobronchitis when hospital admission were much higher than that in control group （P 〈0.05）.It was decreased before discharge and significantly lower than that when hospital admission （P 〈0.05）.There was no different between that in patients before discharge and that in control group （P 〉 0.05）.②The serum level of IFN-γ in patients with acute laryngotracheobronchitis when hospital admission were much lower than that in control group （P 〈0.05）.It was increased before discharge and significantly higher than that when hospital admission （P 〈0.05）.There was no different between that in patients before discharge and that in control group （P 〉0.05）.③The serum level of IgE in patients with acute laryngotracheobronchitis when hospital admission were much higher than that in control group （P 〈 0.05）.It was decreased before discharge and significantly lower than that when hospital admission （P 〈 0.05）.There was no different between that in patients before discharge and that in control group （P 〉 0.05）.Conclusions There is immunologic disorder in infants with acute laryngotracheo-bronchitis.IL-4,IFN-γ and IgE play important role in immunologic pathogenesis.