杨树CDPK基因家族的表达分析及功能预测

Expression and Functional Analysis of CDPK Gene Family in Populus

为探究CDPK基因在木材形成过程中的作用,本研究在基因组水平上对杨树CDPKs基因家族的成员进行分析,找到32个CDPKs及10个CRKs成员。对CDPK基因家族成员在杨树中表达特性进行了分析,发现其家族成员在不同组织、不同发育阶段以及毛白杨次生维管发育不同时期的表达特性不同。分析杨树CDPK基因家族中3个基因Pt CPK1、Pt CPK6、Pt CPK15,其蛋白质一级结构均存在1个跨膜区,其蛋白定位在细胞膜上。

Calcium-dependent protein kinases （CDPKs） are important Ca2^＋ sensors and effectors in Ca2^＋ signal transduction pathway. They play crucial roles in regulation of plant development and tolerance of various environ- mental stresses. In this study, a genome-wide analysis of Populus CDPKs was performed, including phylogeny, gene structure and expression patterns. Thirty-two CDPK genes and ten CRK genes were identified. Furthermore, the gene expression profiles showed that a number of Populus CDPK differentially expressed across different tissues and developmental stages. Three Populus CDPK gene, i.e. PtCPK1, PtCPK6 and PtCPK15, were cloned and ana-lyzed, revealing their proteins localized in cell membrane. These results may lay the foundation for functional analy- sis of Populus CDPK gene family in the future.