多环芳烃暴露与胎儿出生缺陷的相关性研究

Relationship between PAHs exposure and fetal abnormalities

目的探索多环芳烃(PAHs)暴露与胎儿畸形发生的相关性。方法随机选取杭州市37名在医院就诊,并因胎儿畸形行中孕引产的产妇作为研究组,同时选取42名分娩健康新生儿的产妇作为对照,采集母亲外周血和胎儿/新生儿脐带血,用高效液相色谱-荧光检测法测定血清中5种PAHs的含量。依据总PAHs水平,将研究组和对照组进行比较,探讨PAHs暴露水平与不同胎儿畸形之间的相关性。结果母血和脐血血清中Pyrene芘的检出率较高(分别为87.18%和86.33%;DB(a,h)A的检出率最低(分别为10.23%和8.13%)。将研究组和对照组母血和脐血总PAHs暴露水平比较后发现各组间差异无统计学意义(P>0.05)。不同器官畸形分组比较发现,神经管畸形组脐血总PAHs水平较高,与其他组相比差异具有统计学意义(P=0.012)。结论孕期暴露PAHs可能与胎儿出生缺陷的发生有关。

Objective To explore the influence of PAHs exposure on fetal abnormalities. Methods Select 37 pregnant women diagnosed for fetal abnormalities randomly as samples in Hangzhou City,and another 42 pregnant women who gave birth to healthy neonates simultaneously as compared group. The samples of neonatal umbilical cord blood and maternal peripheral blood were collected to detect the concentrations of 5 kinds of PAHs by high performance liquid chromatography- fluorescence detection method. All the results were analyzed according to total concentration of PAHs,so as to explore the influence of PAHs exposure on fetal abnormalities. Results The detection rates of Pyrene in both umbilical cord blood and peripheral blood were high,which was 87. 18% and 86. 33% respectively. The detection rate of DB（ a,h） A was the lowest（ 10. 23% and 8. 13%,respectively）. The statistical analysis after total concentration of PAHs of two groups showed that there was no significant difference（ P 〉0.05）.High total PAHs exposure level of umbilical cord blood were found to correlate with neural tube defects and compared with other groups,here was no statistical difference. Conclusion The intrauterine PAHs exposure may be related to fetal defects.