Beagle犬室性心动过速动物模型的建立

Establishing of ventricular tachycardia animal model in beagle dog

目的研究建立室性心动过速(VT)动物模型的可行性方法。方法成年Beagle犬14只,分为2组,1对照组:4只犬,接受开胸手术,但不结扎左前降支(LAD),2模型组:10只犬,接受开胸法结扎LAD造成急性心肌梗死(AMI)模型,7 d后2组犬均行标准心内电生理检查诱发VT,观察VT诱发、终止情况。结果模型组8/10只犬成功建立AMI模型,术后7 d接受心脏电生理检查,6只模型组犬成功诱发出持续性VT,1只直接诱发出VF,1只诱发出VT便转变为心室扑动、VF,后2只均抢救无效死亡。对照组均未能诱发VT。结论在运用开胸法结扎LAD建立AMI动物模型基础上,通过程序或非程序电刺激方法可成功建立VT动物模型,成功率较高。

Objective To discuss an effective method to establish an animal model of ventricular tachycardia（ VT）. Methods Fourteen healthy adult Beagle dogs were divided into two groups. Ten dogs（ model group） underwent thoracotomy,their left anterior descending（ LAD） coronary arteries were ligated,then acute myocardial infarction（ AMI） was created. After seven days,sustained VT was induced during electrical stimulation. Four dogs（ control group） underwent the same studies exclude the ligation of LAD. Results Eight of ten dogs（ model group） completed AMI model establishment,and underwent an electro-physiological study after 7 days. The sustained VT was induced in 6 dogs of model group,VF induced in 1 dog,non- sustained VT and VF induced in 1dog,the latter 2 dogs died after rescue. And all dogs of control group were unsuccessful. Conclusion The successful rate of establishing an animal model of AMI with ligation of the LAD after thoracotomy is high by programmed electrical stimulation method and non- programmed electrical stimulation method.