前S1蛋白与HBV血清标志物检测结果对比研究

Comparative study of detection results of Pre-S1 protein and HBV serum marker

目的探讨前S1蛋白与乙肝(HBV)血清标志物检测在HBV感染中的临床意义。方法采用酶联免疫吸附试验(ELISA)同时进行乙肝病毒前S1蛋白和HBV血清标志物检测,对比2种检测方法在乙肝检验和治疗中的价值。结果不同HBV感染模式下HBV-DNA检测总阳性率为66.71%,与前S1蛋白检测总阳性59.27%比较,差异有统计学意义(P<0.05);前S1蛋白检测敏感性为81.9%明显高于HBeAg的55.03%(P<0.05),前S1蛋白检测准确性为83.54%高于HBeAg的66.83%(P<0.05)。结论前S1蛋白与HBV血清标志物的检测均能较好的反映乙肝病毒复制情况,前S1蛋白检测敏感性和准确性较高,两者联合测定能为HBV患者提供更为客观的诊断和治疗依据。

Objective To discuss the clinical significance of pre- S1 protein and HBV serum marker in detecting HBV infection. Methods Using the enzyme linked immunosorbent assay（ ELISA） and detecting the pre- S1 protein and HBV serum marker at the same time. Values of the 2 kinds of detection methods in inspection and treatment of HVB were compared. Results Under different HBV infection modes,total positive rate of HBV- DNA detection was 66. 71% obviously higher than pre- S1 protein detection 59. 27%. The difference has statistical significance（ P〈0. 05）. Sensitivity of pre- S1 protein detection was 81. 9% obviously higher than HBeAg 55. 03%（ P〈0. 05）. Accuracy of pre- S1 protein detection was 83. 54% obviously higher than HBeAg 66. 83%（ P〈0. 05）. Conclusion Pre- S1 protein and HBV serum marker detection both can reflect HBV replication,but pre- S1 protein detection has higher sensitivity and accuracy. Combination of the 2 methods can provide more objective diagnostic and treatment evidences for HBV patients.