2013年北京市大兴区麻疹检测分析

Detection and analysis of measles in Daxing District,Beijing,2013

目的分析2013年大兴区麻疹实验室检测结果,了解大兴区麻疹的流行状况,野病毒基因型特征。方法疾控中心采集麻疹疑似病例的血液标本117件,进行麻疹IgM抗体检测;133件咽拭子或尿液标本,以及这些标本的病毒分离物,提取核酸后进行基因分型检测。结果 117件血液标本中,IgM抗体阳性的43件,阳性率36.75%。分型检测产生21株麻疹H1a基因型,占全部核酸阳性的23.08%;4株麻疹D8基因型,占全部核酸阳性的4.40%。结论 2013年大兴区麻疹优势流行基因型为H1a,与北京地区的流行趋势基本相同,D8基因型为北京市新发现的基因型,大兴区出现D8基因型麻疹野毒株,具有一定的研究价值。同时,发现单一的血清学检测分析已经不能全面的说明当地麻疹流行状况,应结合分子生物学技术提高麻疹病原学监测的敏感性。

Objective Analysis of 2013 Daxing District measles laboratory test results,understanding the prevalence of measles in Daxing District and wild virus genotype characteristics. Methods CDC collected blood samples of suspected cases of measles 117,measles IgM antibody to detect; 133 throat swab or urine specimens,these specimens and virus isolates,after extracting the nucleic acid,detect the genotyping. Results 117 blood samples,IgM antibodies was 43,the positive rate was36. 75%. Genotyping produce 21 genotypes of measles H1 a accounted for 23. 08% of all the nucleic acid- positive; 4 measles genotype D8,total positive nucleic acid was 4. 40%. Conclusion 2013 Daxing District advantages measles epidemic genotype was H1 a,and trends are basically the same in Beijing,D8 was newly discovered genotype in Beijing,wild measles virus genotype D8 appeared in Daxing District,which has a certain research value. Simultaneously,a single serological test analysis was found,which was unable to fully explain the prevalence of local measles,and should be combined with molecular biology techniques to improve the monitoring sensitivity of pathogen of measles.